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  • 1980-1984  (17)
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  • 1
    Electronic Resource
    Electronic Resource
    Genes controlling cell proliferation (1980)
    [s.l.] : Nature Publishing Group
    Nature 288 (1980), S. 319-319 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] AN attractive hypothesis for the control of eukaryotic cell proliferation is that it is regulated at a particular point during the G j period before the initiation of DNA replication. This Gj control has been called the 'restriction point' in mammalian cells (Pardee, A. B. Proc. natn. A cad. Sei. ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/288319b0
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  • 2
    Electronic Resource
    Electronic Resource
    High-frequency transformation of the fission yeast Schizosaccharomyces pombe (1981)
    [s.l.] : Nature Publishing Group
    Nature 290 (1981), S. 140-142 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The plasmid, pJDB248, incorporates the S. cerevisiae LEU 2+ gene which codes for -isopropylmalate dehydrogenase, the bacterial plasmid pMB9 and the entire 2 µm plasmid2. It can be propagated in both Escherichia coli and S. cerevisiae, and transforms the leu 2- S. cerevisiae strain MC16 (a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/290140a0
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  • 3
    Electronic Resource
    Electronic Resource
    Cell divison: Coordinating histone transcription and DNA replication (1983)
    [s.l.] : Nature Publishing Group
    Nature 302 (1983), S. 378-378 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] ONE of the more intriguing puzzles posed by the cell division cycle is how the various periodic processes required in the cycle are temporally cordinated. A good example is the coordination of histone supply with DNA replication, since in most eukaryotic cells histone protein synthesis appears to ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/302378a0
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  • 4
    Electronic Resource
    Electronic Resource
    Functionally homologous cell cycle control genes in budding and fission yeast (1982)
    [s.l.] : Nature Publishing Group
    Nature 300 (1982), S. 706-709 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The cdc 2 (previously called wee 2) cell cycle start gene of Schizosaccharomyces pombe, which is required for start and the control of mitosis, has been isolated from an S. pombe gene bank by complementation of a cdc 2 mutation. A functionally homologous sequence which complements the cdc 2 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/300706a0
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular rearrangement of mating-type genes in fission yeast (1982)
    [s.l.] : Nature Publishing Group
    Nature 296 (1982), S. 682-683 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The genetics of the mating-type locus are summarized in Fig. 1. To test this model at the molecular level, we have used the newly developed techniques for genetic transformation of S. pombe4 to isolate a functional copy of the mat-P gene from a gene bank of 5. pombe DNA. The strategy for isolating ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/296682a0
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  • 6
    Electronic Resource
    Electronic Resource
    Gene required in G 1 for commitment to cell cycle and in G 2 for control of mitosis in fission yeast (1981)
    [s.l.] : Nature Publishing Group
    Nature 292 (1981), S. 558-560 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] When dividing S. pombe cells are deprived of nitrogen, they stop proliferating, accumulate in GI and, if they are of appropriate mating type, undergo conjugation and sporulation9'10. To determine whether conjugation is limited to a specific phase of the S. pombe cell cycle, various ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/292558a0
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  • 7
    Electronic Resource
    Electronic Resource
    Cell cycle control : both deterministic and probabilistic? (1980)
    [s.l.] : Nature Publishing Group
    Nature 286 (1980), S. 9-10 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] ONE of the basic questions of cell biology is what determines the rate at which cells undergo division. Currently there are two models of cell cycle control which provide answers to this question. The first is the deterministic growth controlled model which developed mainly from experiments with ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/286009a0
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  • 8
    Electronic Resource
    Electronic Resource
    Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe (1981)
    Springer
    Molecular genetics and genomics 182 (1981), S. 119-124 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A total of 59 new temperature sensitive cdc mutants are described which grow normally at 25°C but become blocked at DNA replication or mitosis when incubated at 36°C. Thirtynine of the mutants are altered in cdc genes which have been identified previously. The remaining 20 mutants define 10 new cdc genes. These have been characterised physiologically, and 6 of the genes (cdc 17, 20, 21, 22, 23, 24) were found to be required for DNA replication, 2 for mitosis (cdc 27, 28), and 2 (cdc 18, 19), could not be unambigously assigned to either DNA replication or mitosis but were definitely required for one or the other. Three genes, the previously identified cdc 10, and cdc 20, 22 are likely to be required for the initiation of DNA replication. Mutants in two genes, cdc 17, 24 undergo bulk DNA synthesis at 36°C, but this DNA is defective. In the case of cdc 17 the defect is in the ligation of Okazaki fragments. cdc 23 is required for bulk DNA synthesis, whilst cdc 21 may possibly be required for the initiation of a particular sub-set of replicons. A previously isolated mutant cdc 13.117 is also further described. This mutant becomes blocked in the middle of mitosis with apparently condensed chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422777
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  • 9
    Electronic Resource
    Electronic Resource
    Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation (1982)
    Springer
    Molecular genetics and genomics 187 (1982), S. 326-329 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A gene bank of partial Sau3A restriction fragments of S. pombe DNA has been constructed in the plasmid vector, pDB248′, which is capable of high frequency transformation of S. pombe. Procedures are described which enable plasmids to be recovered from S. pombe by their reintroduction into E. coli. These methods have been used to detect the S. pombe genes lys 1+, ade 6+ and his 2+ in the gene bank by complementation of mutant gene functions, and to physically isolate the lys 1+ gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331138
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  • 10
    Electronic Resource
    Electronic Resource
    Temperature sensitive allosuppressor mutants of the fission yeast S. pombe influence cell cycle control over mitosis (1984)
    Springer
    Molecular genetics and genomics 196 (1984), S. 332-338 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A collection of Schizosaccharomyces pombe mutants has been obtained which restore activity to a nonsense suppressing tRNA sup3–5 whose suppressing function has been inactivated by second site mutations within the sup3–5 gene. These mutants were screened for those that were temperature sensitive in suppressing the opal nonsense allele ade6-704. Some of these map within or close to sup3 itself and others define two allosuppressor genes sal2 and sal3. The temperature sensitive mutants fail to efficiently suppress any other opal nonsense alleles although one mutant, sup3–5, r57, rr2, weakly does so at the low temperature. sal2 and sal3 mutants have a pleiotropic effect on the cell cycle causing a transient or complete blockage of mitosis. This blockage and the allosuppressor phenotypes are both eliminated by the presence of wee mutations in wee1 or cdc2. Mutants in sal2 are allelic with cdc25, a gene required for successful completion of mitosis. It is suggested that sal3 and cdc25 influence the mechanism that links the growth rate of the cell with the initiation of mitosis. Mutants in these genes may disturb tRNA biosynthesis or protein synthesis and this disruption may have an effect on both nonsense suppression and the growth rate control over mitosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328067
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