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  • 1985-1989  (2)
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  • 1
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Freshly drawn AA and CC red cells were more suitable for in vitro development of Plasmodium falciparum than red blood cells (RBC) stored for 13 days before use. Growth rate inhibition in CC red cell cultures reached 31% in freshly drawn red cells and 57% in aged red cells of the same donor. Ultrastructural studies of CC cells revealed very important irregular cavities sometimes occupied by a granular content. Parasites in CC cells were generally normal but occasionally showed signs of functional impairment. P. falciparum growing in CC red cells was less sensitive in vitro to chloroquine than in AA red cells. This phenomenon may be explained either by the type of the hemoglobin of the host cell or to abnormal haematological parameters of the HbC homozygote donor, particularly the high proportion of neocytes. As metabolism of reduced glutathione is higher in young RBC and as chloroquine lyses parasitized RBC by reducing the regeneration capacities of this compound, the increased rate of young RBC in the CC red cell population was probably related to the decreased chloroquine sensitivity of P. falciparum growing in these cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 92 (1985), S. 141-147 
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Une méthode d'extraction physico-chimique des lipides a été appliquée à des blastospores entières de C. albicans, préalablement à leur étude en microscopie électronique à transmission. Les résultats permettent de supposer une localisation très superficielle des lipides liés à d'autres composants, en particulier peptidopolysaccharidiques, ce qui est en accord avec les données de la littérature. La méthode à également permis l'observation de structures microfibrillaires pariétales. Il s'agit surtout, d'une part, de fibrilles d'environ 50 Å de diamètre, situées dans les couches moyennes, également observables, après mise en oeuvre d'une technique d'extraction de polysaccharides sur cellules entières, et qui ont été assimilées à des fibrilles de ß 1–3 glucanes; d'autre part de fibrilles de 120 À de diamètre, situées au centre du septum de la cicatrice de bourgeonnement et qui ont été assimilées à des fibrilles de chitine.
    Notes: Abstract A chemical method for lipid extraction has been applied to Candida albicans blastospores previously to their examination by transmission electron microscopy. The results led to the concept of a superficial location of lipids bounded to the peptidopolysaccharidic matrix of the cell wall. This lipid extraction also allowed us to describe cell wall microfibrillar structures. Two types of microfibrills have been particularly identified: (1) microfibrils of approximately 50 Å in diameter, involved in a network of the cell wall intermediate layers and supposed to correspond to ß 1–3 glucans; (2) fibrillar structures of 120 Å in diameter, similar to chitin microfibrils, observed in the bud scar septum.
    Type of Medium: Electronic Resource
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