ISSN:
1420-9071
Keywords:
Thiol
;
protein disulfide oxidoreductase (TPOR)
;
insulin
;
disulfides
;
high performance liquid chromatography (HPLC)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A thiol:protein disulfide oxidoreductase from bovine liver was isolated after separation from protein disulfide isomerase. The enzyme, after activation (reduction) with glutathione, was reacted with stoichiometric amounts of insulin and the sulfhydryl groups of the partially reduced hormone were labeled with iodo (l-14C)acetamide. After separation of the insulin chains, the radioactivity was found in both the peptides, with a ratio A-chain/B-chain equal to 2/1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01952081
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