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Folding pathway of guanidine-denatured disulfide-intact wild-type and mutant bovine pancreatic ribonuclease A (1994)

Dodge, Robert W. ; Laity, John H. ; Rothwarf, David M. ; [et al.]

Springer

The protein journal 13 (1994), S. 409-421

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ISSN: 1573-4943

Keywords: Protein folding ; transition-state structure ; ligand binding ; ribonuclease mutant

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Abstract The refolding kinetics of guanidine-denatured disulfide-intact bovine pancreatic ribo-nuclease A (RNase A) and its proline-42-to-alanine mutant (Pro42Ala) have been studied by monitoring tyrosine burial and 2′-cytidine monophosphate (2′CMP) inhibitor binding. The folding rate for wild-type RNase A is faster in the presence of the inhibitor 2′CMP than in its absence, indicating that the transition-state structure in the rate-determining step is stabilized by 2′CMP. The folding rate monitored by 2′CMP binding to the major slow-folding species of Pro42Ala RNase A is faster than the folding rate monitored by tyrosine burial; however, the folding rate monitored by inhibitor binding to the minor slow-folding species is decreased significantly over the folding rate monitored by tyrosine burial, indicating that the major and minor slow-folding species of Pro42Ala fold to the native state with different transition-state conformations in the rate-determining step.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01901697

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Conformation of the ground-state dimer in poly(ethylene terephthalate) (1993)

Dodge, Robert W. ; Mattice, Wayne L.

Bognor Regis [u.a.] : Wiley-Blackwell

Journal of Polymer Science Part B: Polymer Physics 31 (1993), S. 207-212

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ISSN: 0887-6266

Keywords: dimer ; fluorescence ; PET ; poly(ethylene terephthalate) ; Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Notes: Absorbance, excitation, and emission measurements have been performed with methyl benzoate and five model compounds, C6H5COO (CH2)xOOCC6H5, x = 2-6. Under appropriate conditions, three of the model compounds (those with x = 3, 4, 5) show evidence for the formation of intramolecular ground-state dimers. The model compound with x = 5 can form two types of dimers which emit with different energies. The model compound with x = 3 forms one of these dimers, and the model compound with x = 4 prefers the other ground-state dimer. Molecular modeling of the dimers suggests that the two conformations of the ground-state dimers differ in the orientation of the two C=O bonds. In the one dimer these two bonds are nearly parallel, but in the other they make an angle of about 120°. © 1993 John Wiley & Sons, Inc.

Additional Material: 7 Ill.