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Liposome-Mediated DNA uptake by sperm cells (1991)

Bachiller, Daniel ; Schellander, Karl ; Peli, Janos ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Molecular Reproduction and Development 30 (1991), S. 194-200

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ISSN: 1040-452X

Keywords: DNA transfer ; Transgenic animals ; Cationic liposomes ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: To investigate the potential use of sperm cells as vectors to transfer exogenous DNA via the fertilization of oocytes into the germ line of mice, we have used liposomes to transfect DNA into the sperm head. Although the DNA transfer into sperm mediated by liposomes was very efficient and no obvious reduction in the fertilization frequency of oocytes could be detected, we were unable to generate transgenic mice by this method.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/mrd.1080300305

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