Electronic Resource
Springer
Methods in cell science
13 (1991), S. 285-288
ISSN:
1573-0603
Keywords:
DNA
;
fluorimetry
;
fibroblast
;
cell culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary An improved direct fluorimetric assay is described using bisbenzimidazol fluorescence at 356 nm excitation and 458 nm emission wavelengths. The turbidity of the medium was shown to have no effect on fluorescence under an absorbance of 0.2 at the excitation wavelength. The method was evaluated by comparisons with a colorimetric DNA assay and cell counting. The linearity of fluorescence was determined up to 15μg/ml of DNA. The method was very reproducible and sensitive to detect 10 ng/ml of DNA and may be used for cell suspensions containing around 5000 cells/ml or more.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02388262
Permalink
|
Location |
Call Number |
Expected |
Availability |
