ISSN:
0951-4198
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
The use of ultraviolet matrix-assisted laser desorption (MALD) to ionize peptides for analysis in a quadrupole ion trap is described. An ion source was modified to accommodate a fiber optic to transmit laser radiation from a notrogen laser (337 nm) to the tip of the sample probe containing peptide of protein samples in a matrix of 2,5-dihydroxybenzoic acid (DHB) or 3,4-dimethoxy-4-hydrexy-cinnamic acid. Detection limits are demonstrated with 10 fmol of sperm-whale-myoglobin. The dimer of sperm-whale myoglobin was also observed at m/z 34, 430. A. comparison is made of the tandem mass spectrum of (MS/MS) of human angiotensin I desorbed by MALD to that of the peptide desorbed by liquid secondary-ion mass spectrometry. Both spectra were found to contain abundant structural information.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/rcm.1290070106
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