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Detection of antibodies against corrosion products in patients after Co—Cr total joint replacements (1994)

Yang, Jun ; Merritt, Katharine

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 28 (1994), S. 1249-1258

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ISSN: 0021-9304

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine , Technology

Notes: Sensitivity to nickel, cobalt, and chromium is common among the general population. The identification of these sensitivities is generally by the detection of cell-mediated immunity. Detection of humoral immunity to these metals has rarely been undertaken because suitable assays are not available. To further our knowledge about the systemic humoral immune system response to weak hapten-systemic or allogeneic protein conjugates (corrosion and wear products of metallic orthopedic devices), a sensitive enzymelinked immunosorbent assay (ELISA) method for testing for antibody (humoral immunity) to metals was developed. Microtiter plates were coated with human serum albumin (HSA) or glutathione (GSH) and then conjugated with metal ions. Plates coated with HSA or GSH and without metal ions served as background controls. An indirect ELISA method was used to detect antibody binding. Patients bearing cobalt-chromium alloy devices were considered to be the most likely candidates as sources of antibody to metal and were used to document the usefulness of the test. Specificity of the test was documented by inhibition studies with these metal ions in the ELISA assay. The serum samples of 10 patients were screened for the presence of Cr-, Co-, or Ni-specific antibody at various periods up to 5.5 years after implantation of F-75 cobalt alloy total joint replacements. All patients had implants that were functioning well. The pre- and postimplantation humoral immune profiles of patients were determined and compared with each preimplantation control and with a pooled normal serum sample. This study presents evidence that metalprotein complex-specific antibodies can be detected. The clinical implications remain to be determined. © 1994 John Wiley & Sons, Inc.

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jbm.820281102

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Decreased neutrophil respiratory burst on exposure to cobalt-chrome alloy and polystyrene in vitro (1992)

Shanbhag, Arun ; Yang, Jun ; Lilien, Jack ; [et al.]

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 26 (1992), S. 185-195

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ISSN: 0021-9304

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine , Technology

Notes: The effect of biomaterials on the super-oxide-producing ability of neutrophils was studied. Human peripheral blood neutrophils were incubated with cobalt-base alloy (F-75) or polystyrene beads of nonphagocytosable size. Respiratory burst activity was studied by measuring super- oxide dismutase inhibitable reduction of cytochrome C. Neutrophils were found to release no more superoxide anion on incubation for up to 3 h with either material in a protein-free medium than controls without foreign material. However, the ability of neutrophils incubated with either biomaterial to subsequently respond to phorbol myristate acetate challenge was decreased (p 〈 0.05). Chemical analysis of supernatants for the F-75 samples showed a high concentration of cobalt in the medium within 1 h of incubation. Minimal chromium and nickel was detected. No correlation could be demonstrated between metal in solution and the respiratory burst defect in neutrophils. Instead it appears that interaction of cells with either surface was the critical event in altering the response to phorbol myristate acetate. This observed functional defect may play an important role in rendering tissue around implanted biomaterials susceptible to infections.

Additional Material: 4 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jbm.820260205

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Human neutrophil response to short-term exposure to F-75 cobalt-based alloy (1992)

Yang, Jun ; Shanbhag, Arun ; Lilien, Jack ; [et al.]

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 26 (1992), S. 1217-1230

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ISSN: 0021-9304

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine , Technology

Notes: The effect of biomaterials on the activation of human neutrophils was studied. Human neutrophils were incubated with F-75 cobalt-based alloy or polystyrene microspheres of a nonphagocytosable size with two times total neutrophil plane surface area. Scanning and transmission electron microscope (SEM, TEM), energy dispersive x-ray microanalysis (EDX), and graphite furnace atomic absorption spectroscopy (GFAAS) were used to analyze changes in cellular morphology and metal content. This report presents evidence that human PMNs display morphological changes related to foreign material challenge, including activation on F-75 bead surfaces, pinocytosis of corrosion products, formation of intracellular vacuoles, degranulation, etc. Moreover, when PMNs were present, the corrosion release rate of F-75 increased as much as three times over cell-free controls. © 1992 John Wiley & Sons, Inc.

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jbm.820260911

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