ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The active non-glycosylated glucoamylase, overexpressed from the Saccharomycopsis fibuligera GLU1 gene in Escherichia coli BL21(DE3), has been purified from the solubilized inclusion bodies and then renatured in vitro. Crystals of the recombinant glucoamylase were obtained by vapour diffusion using PEG as precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell dimensions of a = 58.1, b = 87.8 and c = 99.9 Å, and diffract to 1.7 Å resolution. This is the first report of the crystallization of the full-length glucoamylase corresponding to the mature enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444997006835
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