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  • 1970-1974  (14)
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  • 1
    Electronic Resource
    Electronic Resource
    Carbohydrate-binding proteins from the sieve-tube sap of Robinia pseudoacacia L. (1974)
    Springer
    Planta 121 (1974), S. 197-200 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Evidence is provided for the occurrence of lectins (phytohemagglutinins) in the sieve-tube sap of Robinia pseudoacacia L. The most potent sugars found to inhibit the complex formation of the soluble protein with rabbit erythrocytes were N-acetyl-galactosamine and glycosides containing galactose. Sucrose and inositol had weaker effects. The extract of the membraneous material present in the sieve-tube sap also contains lectins, but the sugar specifity is different.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388759
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  • 2
    Electronic Resource
    Electronic Resource
    Unterschiede in der Lichtaktivierung der NADP-abhängigen Glycerinaldehyd-3-phosphat-Dehydrogenase und der Ribulose-5-phosphat-Kinase bei Pflanzen des Calvin- und des C4-Dicarbonsäure-Fixierungstypus (1971)
    Springer
    Planta 96 (1971), S. 109-118 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Preceding experiments had shown that irradiance of intact leaves or of isolated chloroplasts causes a reversible increase in the activity of NADP-GPD (Ziegler and Ziegler, 1965) as well as of ribulose-5-phosphate kinase (Latzko and Gibbs, 1969). Examination of several species which carry out the Calvin type of photosynthetic CO2 fixation (Vicia faba, Spinacia oleracea, Nicotiana tabacum, Avena sativa) now revealed that the dark level of NADP-GPD activity ranges between 300–400 μmol NADPH/mg chlorophyll·h; irradiance causes an activation to an turnover rate of 900–1600 μmol NADPH/mg chlorophyll·h. 2. The dark-level of ribulose-5-phosphate kinase in these Calvin type plants corresponds to about 400 \gmmol PO4---/mg chlorophyll\sdh. It rises to 900\2-1300 \gmmol PO4---/mg chlorophyll\sdh after irradiance. 3. In all species examined which carry out the C4-dicarboxylic acid type of CO2 fixation (Zea mays, Cyperus rotundus, Portulacca oleracea, Saccharum officinarum) the dark-level of NADP-GPD as well as of ribulose-5-phosphate kinase is already as high as the light-level of Calvin type plants. In these species irradiance either activates both enzymes only to a small extent (Saccharum officinarum, Portulacea oleracea) or it activates only one of the two enzymes to an exceptional high activity (NADP-GPD in Zea mays, ribulose-5-phosphate kinase in Cyperus rotundus), while the activity of the other one remains nearly constant. 4. The dark-level of NADP-GPD in young Zea mays (2 leaves expanded) is as high as in adult plants; moreover its further activation by light corresponds to that in adult plants. In contrast, the dark-activity of ribulose-5-phosphate kinase in young Zea mays corresponds to the lower level found in Calvin type plants and is activated by irradiance in the same manner as it is in the latter plants. 5. The activity of ribose-5-phosphate isomerase is not influenced by light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386361
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  • 3
    Electronic Resource
    Electronic Resource
    Enzymaktivitäten im Siebröhrensaft von Robinia pseudoacacia L. und anderer Baumarten (1971)
    Springer
    Planta 98 (1971), S. 330-356 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Electron microscopic studies of the sieve tube sap obtained from the secondary phloem of Robinia pseudoacacia by the method of Hartig (1860) showed the presence of well developed mitochondria in addition to membrane fragments. 2. In this sieve tube sap the following enzymes could be detected qualitatively: UTP-glucose-1-phosphate-uridyl transferase, UDPG-fructose glucosyl transferase, glucose-6-phosphate dehydrogenase, hexokinase (for glucose and fructose), phosphohexose isomerase, phosphofructokinase, and UDPG-pyrophosphatase. 3. The following enzymes were determined quantitatively: phosphorylase, amylase, aldolase, triosephosphate isomerase, NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase, phosphoglyceromutase, enolase, pyruvate kinase, pyruvate decarboxylase, alcohol dehydrogenase, isocitrate dehydrogenase, fumarase, malate dehydrogenase, glutamate-pyruvate transaminase, glutamate dehydrogenase, glutamate-oxalacetate transaminase, and anorganic pyrophosphatase. 4. The following enzymes could not be detected: UDGP dehydrogenase, UDPG-fructose-6-phosphate-glucosyltransferase, invertase, phosphoglucomutase, lactate dehydrogenase, and citrate synthase. 5. The enzyme pattern in the sieve tube saps of Tilia platyphyllos, Carpinus betulus, Fraxinus americana, Quercus borealis maxima, and Salix viminalis is qualitatively similar to that of Robinia, but shows quantitative differences (as far as analyzed). 6. The meaning of the results for the metabolism and function of the sieve tubes in situ is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380234
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  • 4
    Electronic Resource
    Electronic Resource
    Der Einbau von 32PO4 --- in organische Verbindungen durch Siebröhrensaft (1971)
    Springer
    Planta 99 (1971), S. 154-162 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Phloem exudates collected from the secondary phloem of Tilia platyphyllos, from the flower stalks of Yucca flaccida and from stems of Salix triandra (obtained by the aphid method) were incubated with 32PO4 ---. The radioactivity was incorporated into a series of organic compounds very similar to those synthesized after the whole phloem is incubated with 32PO4 ---. This synthetic ability of the exudate was completely destroyed by heating; therefore it is very probably due to enzymes. 2. ATP is one of the very first compounds labeled after incubation of the sieve tube sap with 32PO4 ---. It already shows radioactivity after 10 sec. 3. The phloem exudate of Yucca flaccida does not lose the synthetic ability even after several hours of secretion. This indicates that the enzymes and the substrates for the phosphorylations are secreted continuously into the lumen of the sieve tubes. 4. The formation of the organic phosphorus compounds in the sieve tube sap is in accordance with the results of the enzyme analyses in this material (Kennecke et al., 1971).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388247
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  • 5
    Electronic Resource
    Electronic Resource
    Untersuchungen über ATP und andere organische Phosphorverbindungen im Siebröhrensaft von Yucca flaccida und Salix triandra (1970)
    Springer
    Planta 91 (1970), S. 68-79 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Like the sieve tube sap of dicotyledons analysed so far, the phloem exudate of Yucca flaccida contains a high level of ATP, i.e. 615 μg/ml on the average. ATP concentration in the phloem exudate does not decrease during extended periods of exudation. 2. The pattern of labelled phosphorylated compounds after feeding of 32PO 4 --- is essentially similar in the phloem exudate of Yucca flaccida and in the exudate from the mouth parts of aphids feeding on Salix triandra phloem. 3. The label was incorporated very rapidly into most of the phosphorylated organic compounds present in the exudate of Yucca flaccida. in the first phase after feeding of 32PO 4 --- only the label in fructose-1,6-diphosphate and possibly the label in ATP was still increasing in relation to the total radioactivity. This increment was correlated to a loss of radioactivity in the inorganic phosphate fraction. 4. Specific radioactivity of inorganic phosphate and ATP in the sieve tube sap of Yucca flaccida increased continuously throughout the experiments while 32PO 4 − was administered externally. This trend continued for some time after removal of the external source of label.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390166
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  • 6
    Electronic Resource
    Electronic Resource
    Förderung der Hill-Reaktion und der CO2-Fixierung in isolierten Spinatchloroplasten durch niedere Sulfitkonzentrationen (1972)
    Springer
    Planta 109 (1972), S. 269-279 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. The photoreduction of K3[Fe(CN)6] by isolated and sonicated spinach chloroplasts is increased by SO 3 -- (concentrations tested: 0.25–5 mM). This stimulation increases with SO 3 -- concentrations from 0.25–3 mM. The ferricyanide-reduction with SO 3 -- is inhibited by DCMU (10-6M) to about 90%. Inhibition of the photoreduction by pretreatment of the chloroplasts with Tris-buffer is compensated by increasing concentrations of SO 3 -- (tested up to 3 mM). 2. The photoreduction of NADP in isolated chloroplasts is also enhanced by SO 3 -- (concentrations tested: 0.25–3.0 mM). It is completely inhibited by DCMU (10-6M). In contrast to the results with ferricyanide as electron acceptor, SO 3 -- does not overcome the inhibition of NADP reduction caused by pretreatment of the chloroplasts with Tris-buffer. 3. In illuminated isolated chloroplasts SO 3 -- concentrations 〈1 mM do not alter the ATP-concentration, concentrations〉1 mM decrease it. 4. The photosynthetic fixation of 14CO2 by isolated chloroplasts is increased by SO 3 -- concentrations 〈1 mM, but decreased by concentrations〉1 mM. In total 14CO2 fixed, at stimulating concentrations (0.25 and 0.5 mM SO 3 -- ), the relative amount of sugar-monophosphates is increased, whereas that of sugardiphosphates and of PGA is decreased. 5. It is proposed that these specific effects on photosynthetic processes help to explain the well known fact that photosynthetic gas exchange and the yield of plants are stimulated by low doses of SO2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387089
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  • 7
    Electronic Resource
    Electronic Resource
    Elektronenmikroskopische Untersuchungen SO2-begaster BlÄtter vonVicia faba (1973)
    Springer
    Protoplasma 76 (1973), S. 83-96 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung BlÄtter vonVicia faba, die noch mit der Pflanze verbunden waren, wurden einzeln mit SO2 unter konstanten physikalischen Bedingungen begast und die VerÄnderungen der Chloroplasten im Schwammparenchym akut geschÄdigter BlÄtter untersucht. Als erste VerÄnderungen an den Chloroplasten wurden Granulierung des Stromas und AufblÄhungen der Stroma- und Granathylakoide festgestellt. Im Laufe der SchÄdigung nahm das Chloroplastenvolumen gegenüber den Kontrollen erheblich zu. Unterschiede im Durchmesser und in der Anzahl der osmiophilen Plastoglobuli konnten nicht statistisch gesichert werden. Im letzten Stadium der akuten SchÄdigung löste sich zuerst die Plastidenhülle auf, die AufblÄhungen der Thylakoide verschwanden wieder. Die Thylakoidmembranen blieben bemerkenswert lange erhalten, zeigten aber dann starke Auflösungserscheinungen. Die elektronenoptische Dichte nahm in der letzten beobachteten SchÄdigungsphase auf Grund einer einsetzenden Schrumpfung der Chloroplasten zu.
    Notes: Summary Leaves ofVicia faba, still attached to the plant, were individually exposed to SO2, under constant physical conditions. The changes in the chloroplasts of the spongy parenchyma of acutely damaged leaves were examined. The first modifications in the chloroplasts were the granulation of the stroma and the swelling of the stroma- and grana-thylakoids. Subsequently the volume of the chloroplasts increased considerably. It was not possible to obtain statistically significant differences in diameter or number of osmiophilic plastoglobuli. In the final stage of acute damage the plastid membrane dissolved and then the swelling of the thylakoids disappeared. The thylakoid membranes remained intact for a remarkably long time, but then showed very clear symptoms of decomposition. In the final phase of damage the electron density increased because the chloroplasts had begun to shrink.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279674
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  • 8
    Electronic Resource
    Electronic Resource
    Erratum (1973)
    Springer
    Protoplasma 76 (1973), S. 282-282 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280704
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  • 9
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    Untersuchungen �ber ATP und andere organische Phosphorverbindungen im Siebr�hrensaft von Yucca flaccida und Salix triandra (1970)
    Springer
    Details
    Publication Date: 1970-01-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 10
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    Carbohydrate-binding proteins from the sieve-tube sap of Robinia pseudoacacia L. (1974)
    Springer
    Details
    Publication Date: 1974-01-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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