Publication Date:
1996-12-06
Description:
Pharmacological studies support the idea that nitric oxide (NO) serves as a retrograde messenger during long-term potentiation (LTP) in area CA1 of the hippocampus. Mice with a defective form of the gene for neuronal NO synthase (nNOS), however, exhibit normal LTP. The myristoyl protein endothelial NOS (eNOS) is present in the dendrites of CA1 neurons. Recombinant adenovirus vectors containing either a truncated eNOS (a putative dominant negative) or an eNOS fused to a transmembrane protein were used to demonstrate that membrane-targeted eNOS is required for LTP. The membrane localization of eNOS may optimally position the enzyme both to respond to Ca2+ influx and to release NO into the extracellular space during LTP induction.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kantor, D B -- Lanzrein, M -- Stary, S J -- Sandoval, G M -- Smith, W B -- Sullivan, B M -- Davidson, N -- Schuman, E M -- 49176/PHS HHS/ -- NS37292/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1996 Dec 6;274(5293):1744-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Biology 216-76, California Institute of Technology, Pasadena, CA 91125, USA. schumane@cco.caltech.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8939872" target="_blank"〉PubMed〈/a〉
Keywords:
Adenoviridae/genetics
;
Animals
;
CHO Cells
;
Cell Membrane/enzymology
;
Cricetinae
;
Cytosol/enzymology
;
Endothelium/*enzymology
;
Genetic Vectors
;
Hippocampus/*physiology
;
In Vitro Techniques
;
*Long-Term Potentiation/drug effects
;
Mice
;
Myristic Acid
;
Myristic Acids/metabolism/pharmacology
;
Neurons/*physiology
;
Nitric Oxide Synthase/genetics/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
Synaptic Transmission
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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