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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A complete set of nine primary trisomics (2n+ 1) for cv.‘Yugu No. 1’of foxtail millet, Setaria italica (L.) Beauv. (2n= 2x= 18), was identified cytologically from progenies derived from crosses between autotriploids(2n= 2x= 27) and their diploid counterparts. Five autotriploid plants were identified from 2100 seedlings derived from 4x-2x crosses; the reciprocal crosses (2x-4x) failed to produce autotriploids. Autotriploids grew vigorously and were morphologically very similar to diploids. Theprimary trisomics (2n= 2x= 19) constituted ≅32.5% of the total progeny from the 3x-2x crosses, whereas 59.8% of the descendants were aneuploids with chromosome numbers ranging from 20 (double trisomics and tetrasomics) to 37 (2n= 4x+ 1; or autotetraploid with one additional chromosome). The nine primary trisomics identified were self-fertile; seven had characteristic morphology, whereas trisomics VIII and IX resembled the disomics. The seed set for trisomic V was the lowest (20%), and trisomic VIII the highest (74%). Other aneuploids with 20 or more somatic chromosomes were either self-sterile or partially fertile with various, but low, levels of seed set. Each of the primary trisomics showed its unique transmission rate when self-pollinated; trisomic IX had the highest (45.8%), whereas trisomic V had the lowest (19.6%) transmission rate.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 116 (1997), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In order to breed yellow-seeded rapeseed, 16 yellow-seeded lines of Brassica napus L. derived from eight genetic sources were used. The genetic variation of the seedcoat ratio, the cellulose content of the seedcoat, the oil content of the seedcoat and of the embryo, and also the correlations between these characters of the yellow- and brown-seeded plants from the same line, were analysed by variance analysis and path analysis. The results show that the seedcoat ratio and cellulose content of brown seeds are 4.2% and 17.74%, respectively, higher than that of yellow seeds and the oil content of the seedcoat of brown seeds is 3% lower than that of the yellow seeds, these differences all being highly significant. However, the differences between yellow and brown seeds in 1000-seed weight and oil content of the embryo were very small. Both characters are determined mainly by the genetic background and not by seed colour or seedcoat thickness. The correlation analysis revealed that the seedcoat thickness has a highly significant positive correlation with the cellulose content of the seedcoat and is highly significantly negatively correlated with the seedcoat oil content and the 1000-seed weight. The oil content of the embryo alone has a highly significant negative correlation with 1000-seed weight. In yellow seeds, the seedcoat thickness has a large and directly positive effect on the oil content of the embryo whereas the 1000-seed weight has a negative one; the opposite was found in brown seeds. Selection objectives in breeding yellow seeds in Brassica napus are also discussed.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Geophysical journal international 116 (1994), S. 0 
    ISSN: 1365-246X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: In vertical seismic profiling tube waves are generally classified as noise. They have, however, useful applications: tube waves can be generated by compressional waves at fluid-filled cracks crossing the wall of the borehole. In this case the permeability of the fracture zone can be estimated from the amplitude ratio of the incident P wave and the generated tube wave.In the present study an improved model for tube-wave amplitude interpretation is presented. While former models assumed isolated cracks, now, the consideration of fracture zones of a certain porosity, permeability and width is allowed. Master curves are used to check the sensitivity of the amplitude ratio of tube and P waves with respect to the formal parameters of computation. It is shown, moreover, that mainly those parts of a fracture contribute to the tube-wave generation which are within a radius of about 1 m of the borehole.The problem of localization and characterization of fracture zones by tube waves was investigated with several complementary field experiments in shallow boreholes in crystalline rocks as vertical seismic profiling (VSP) with three-component borehole geophones and hydrophones, televiewer measurements, well tests and temperature measurements.It turned out that fracture zones were met at all depths where tube waves were generated. However, not all of them were found to be hydraulically activated during well tests and temperature measurements. This means that fractures detected by tube-wave VSP are not necessarily of hydrological importance. Transmissivity values based on tube-wave analysis are, therefore, an upper limit for the well under consideration. On the other hand, the transmissivity values derived from the well tests agreed quite well with those of the tube-wave analysis if only the ‘open’fracture zones were considered: a fact which seems to confirm our theoretical model.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 17 (1994), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Purification and in vitro cultivation techniques were developed for the fish haemoflagellate, Trypanosoma danilewskyi. The parasites were isolated and purified from the peripheral blood of experimentally infected goldfish, using a combination of Ficolt-paque gradient centrifugation to remove fish red blood cells and in vitro incubation to remove the remaining fish leucocytes. A serum-free culture medium for T. danilewskyi supported both short- and long-term cultivation of the haemoflagellates. The serum-free medium is a mixture of reagents available commercially: Leibovitz's L-15 medium, Dulbecco's Modified Eagle Medium and Hank's balanced salt solution. The doubling time was calculated to be 44.4 × 7.8h. Typically, a two- to five-fold increase in the number of cultured parasites was observed on day 7 after subculture with 1 × 106 and 5 × 105 trypanosomes ml−1, respectively. When administered to fish, the in vitro-derived parasites caused an infection and pathology whose characteristics were similar to those observed following infection with trypanosomes obtained from infected goldfish. The freshly isolated and in vitro-grown parasites were successfully cryoprescrvcd in the culture medium containing 10% glycerine at −80°C for at least 3 months. Although the viability of the parasite decreased by 40–50% after thawing, cryoprcserved parasites retained the ability to infect goldfish.Correspondence: Dr M. Belosevic, Associate Professor, Department of Zoology, CW-312 Biological Sciences Building, University of Alberta, Edmonton, AB, Canada T6G 2E9.
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