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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 750 (1995), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Westerville, Ohio : American Ceramics Society
    Journal of the American Ceramic Society 80 (1997), S. 0 
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The elastic properties of unidirectional and 0°/90° crossply Nicalon-SiC-fiber-reinforced calcium aluminosilicate (CAS/SiC) ceramic-matrix composites have been measured using a resonant ultrasound spectroscopy (RUS) technique. This approach has allowed the nondestructive determination of the complete set of independent second-order elastic stiffness constants of these ceramic composites. These stiffness data have been used to obtain the orientation dependence of Young's modulus and the shear modulus. The results are in reasonably good agreement with the limited experimental data obtained from mechanical testing. The RUS measurements reveal that the unidirectional CAS/SiC composite is well modeled by transverse isotropic symmetry, indicating relatively isotropic fiber spacing in the transverse plane. The analysis indicates that the overall elastic anisotropy is also small for unidirectional and 0°/90° laminated CAS ceramic-matrix composites, a result that can be attributed to the relatively low modulus ratio of the Nicalon SiC fiber to the CAS matrix and to the moderate fiber volume fraction.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 93 (1995), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effects of chemical fixation on tip morphology and immunolocalization of the cytoskeleton in Tradescantia virginiana pollen tubes were evaluated using lour different fixation protocols differing in fixative type/concentration, fixation time, buffer system and additives. Apical regions were much more sensitive to fixation manipulations than more basipetal areas. The presence of the calcium chelator EGTA at 5 mM led to tip rupture and/or swelling in over 80% of germinated grains. However, low or no EGTA levels during fixation resulted in poor immunolocalizations, although lips had more normal morphology. Double fixation in which pollen tubes were first treated for a short period with higher fixative and lower EGTA (0.5 mM) concentrations, followed by lower fixative and higher EGTA (5.0 mM) concentrations, resulted in both improved preservation of pollen lube tip morphology and microfilament/microtubule localizations.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 96 (1996), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The inhibitory effects of benomyl on pollen tube growth have received little attention, particularly at the microscopic and immunohistochemical levels. Pollen germination and tube growth in the presence of benomyl were evaluated in Tradescantia virginiana to investigate the effects of this fungicide on pollen germination rate, tube growth and morphology, and microtubule (Mt) organization. Benomyl was incorporated in germination media at 0,480, 600 or 720 mg 1−1. Inhibition of pollen germination, cytoplasmic streaming, and tube elongation were associated with benomyl treatments. Benomyl also induced abnormal pollen tube morphology and Mt organization. Compared to controls, Mts in the treated tubes were characteristically fewer in number, fragmented, sinuous and increasingly disorganized. At the two highest benomyl concentrations, Mts were considerably fewer or absent in apical/subapical regions of the pollen tubes. This work verifies that benomyl incorporated into germination media at concentrations lower than recommended field rates inhibit pollen germination and tube growth, and that the effects are associated with alterations of Mt organization.
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  • 6
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 400-402 (Oct. 2008), p. 257-262 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: According to the spatial distribution theory of damping energy dissipation, the calculation formula of damping energy dissipation and damping ratio of a cantilever beam in one cycle are derived. It is shown that the damping characteristic of a member is affected by the material damping characteristic, stiffness distribution, mode of vibration and the sectional shape of the member, etc. The damping energy dissipation coefficient J for reinforced concrete materials is calculated by using the proposed formula considering different concrete strength, elastic modulus and reinforcement ratios based on test data available
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  • 7
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 561-565 (Oct. 2007), p. 45-48 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The samples of V-N microalloyed mild steel were obtained in duo mill with the normaldeformation rate and the normal rolling temperature except final rolling temperature, which is at780°C, 730°C and 680°C respectively. The tensile test was carried out and the microstructure wasobserved with optical microscope. It was found that the mechanical properties improved dramaticallycompared with normal process, the final rolling temperature is more than 900°C. The main reason isthe fine grain size and second phase particles. With the final rolling temperature decreasing, themechanical property further improves until the final rolling temperature of 700°C
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-2145
    Keywords: Microfilament ; Microtubule ; Pollen tube growth ; Propiconazole ; Tradescantia virginiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of propiconazole on germination and tube growth of Tradescantia virginiana pollen when incorporated in germination media at 0, 102, 136, or 170 μl l−1 were evaluated using light microscopy and immunocytochemistry. Propiconazole inhibited pollen germination, cytoplasmic streaming, and tube elongation. Treatments also induced abnormal tube morphology and cytoskeletal distribution. Tubes treated with propiconazole displayed weaker microfilament (Mf) signals along the pollen tubes, with amorphous staining. Microtubule (Mt) distribution was also severely affected. In treated tubes, the proximal portions had characteristically fragmented Mts. Fewer Mt bundles were seen in the subapical region, and these were located further from the apex. Propiconazole effects were generally concentration dependent. The results indicate that propiconazole affects both Mfs and Mts; however, the effects may be an indirect result of the drug's influence on membranes.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 15 (1996), S. 1392-1394 
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-0603
    Keywords: Freeze substitution/fixation ; Immunofluorescence localization ; Microtubules ; Pollen tubes ; Tobacco or mouse suspension cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunofluorescence localization is widely used to determine the distribution and organization of the microtubule (Mt) cytoskeleton in cells, with most protocols using conventional chemical fixation (CCF). However, concerns associated with CCF are slow rate of fixation, alteration of cell morphology, and redistribution of target antigens. Freeze substitution/fixation (FSF) is a method commonly used in electron microscopy to overcome these problems, but has not been exploited at the light microscopic level. Thus, techniques using FSF were investigated in three divergent cell types (tobacco suspension cultures, Tradescantia pollen tubes, and mouse myeloma cultures) and evaluations were made of cell morphology and immunofluorescence localization of the Mt cytoskeleton. Tobacco cells prepared by FSF exhibited an extensive network of Mts with consistent and strong labeling, while CCF preparations had disorganized and fuzzy Mts. Pollen prepared with FSF retained cytoplasmic details, and had continuous longitudinal arrays of cortical Mts. In contrast, CCF induced structural abnormalities in pollen characterized by swollen and ruptured tube tips with sinuous, nonuniformly labeled Mts. Likewise, in myeloma cells, Mt labeling was more uniform and stronger with less background with FSF versus CCF. CCF also caused myelomas to shrink. Advantages of FSF over CCF are that rapid freezing attains an instantaneous cessation of metabolism with better preservation of cellular structures, and enhanced and more consistent Mt labeling. The absence of numerous amendments used in CCF (e.g., buffers and osmotic reagents) may avoid interaction with target antigens. FSF preparations should be useful in critical immunofluorescence localizations of other antigens, especially those sensitive to CCF manipulations.
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