Publication Date:
1990-02-09
Description:
The role of exocytosis of major histocompatibility complex (MHC) class I molecules in the presentation of antigens to mouse cytotoxic T lymphocytes (CTLs) was examined by use of a recombinant vaccinia virus that expresses the E19 glycoprotein from adenovirus. E19 blocked the presentation of vaccinia and influenza virus proteins to CTLs in a MHC class I allele-specific manner identical to its inhibition of MHC class I transport from the endoplasmic reticulum. This finding indicates that (i) the relevant parameter for antigen presentation is the rate of MHC class I molecule exocytosis, not the level of class I cell surface expression, and (ii) association of class I molecules with antigen is likely to occur within the endoplasmic reticulum.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cox, J H -- Yewdell, J W -- Eisenlohr, L C -- Johnson, P R -- Bennink, J R -- New York, N.Y. -- Science. 1990 Feb 9;247(4943):715-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2137259" target="_blank"〉PubMed〈/a〉
Keywords:
Adenovirus Early Proteins
;
Animals
;
Antigen-Presenting Cells/drug effects/immunology/*ultrastructure
;
Antigens/*immunology
;
Antigens, Viral/immunology
;
Antiviral Agents
;
Biological Transport
;
Brefeldin A
;
Cell Line
;
Cyclopentanes/pharmacology
;
Endoplasmic Reticulum/*immunology
;
Exocytosis
;
Glycoproteins/analysis
;
H-2 Antigens/immunology
;
Histocompatibility Antigens Class I/immunology/*metabolism
;
Mice
;
Mice, Inbred A
;
Mice, Inbred BALB C
;
Mice, Inbred C3H
;
Mice, Inbred CBA
;
Oncogene Proteins, Viral/analysis
;
Orthomyxoviridae Infections/immunology
;
Recombinant Proteins/immunology
;
T-Lymphocytes, Cytotoxic/*immunology
;
Vaccinia virus/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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