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  • 1
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    In:  http://aquaticcommons.org/id/eprint/22541 | 18721 | 2018-04-23 11:20:39 | 22541 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-07
    Description: In this research the affect of A. hydrophila on pathogenesis of Crayfish A. leptodactylus was carried out. This study was designed in three groups as treatments and one group as control in triplicate with 20 Crayfish in each glass aquarium. The treatments have exposed to A. hydrophila. The concentrations of the bacteria in treatments were , and CFUmL-1 respectively. The untreated control group was disinfected by oxytetracyclin at concentration of 100 ppm for 24 hours. The haemolymph samples were withdrawn for measuring of THC and TPC within interval hours (2, 6, 12, 24, 48, 96, 144, 240 and 336). The Crayfish samples were then fixed in Davidson fixative. The results showed that the differences of THC value between treatment 4 (95.33±23.16) and control (1.13±.45) were significant (P〈0.05) as well as control and treatments 3 (35.33±16.16) and 4 (95.33±23.16) during 2 initial hours. In case of THC the significant difference (P〈0.05) was observed between control group (72.33±15.04) and treatment 3 (13.13±5.85) after 48 hours. During 240 hours, also the difference of THC value between control group (80.33±24.94) with treatment 2 (14.67±3.51) was significant (P〈0.05). The finding of TPP value showed that there was no significant difference between control group and treatments in during mentioned times (P〈0.05). The result of histopathology in hepatopancreas and gill sections showed that hemocyte aggregation and necrosis within pyknosis of nucleus. In lower concentrations of bacteria no pathological changes of heart were observed. In digestive tract no changes were appeared in treatments, but in concentration of 3×108 CFU ml-1 a low aggregation of hemocytes was revealed.
    Keywords: Biology ; Fisheries ; Astacus leptodactylus ; Aeromonas hydrophilia ; Total Hemocyte Count ; Total Protein Concentration ; Histopathology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
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    Format: 644-656
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  • 2
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    In:  http://aquaticcommons.org/id/eprint/22747 | 18721 | 2018-05-19 18:40:13 | 22747 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-09
    Description: White spot syndrome virus (WSSV), the causative virus of disease, is found in most shrimp farming areas in Iran, and it causes large economic losses to the shrimp farming industry. Shrimp aquaculture is an important industry in Iran and plays an important role in aquaculture production. The shrimp production in 2012 was more than 12000 metric tonnes and it is estimated it will reach 20000 tonnes in 2013. White spot syndrome virus is highly virulent in shrimp farms and can spread quickly and cause up to 100% mortality within 3-7 days. The virus is a very large, enveloped, double stranded DNA (ds DNA) and assigned by ICTV to a new genus Whispovirus and belong to Nimaviridae family. In Iran WSSV first appeared in Khuzestan Province in the south of Iran, and later on it appeared in other provinces such as Bushehr, and Sistan and Baluchestan. The aim of this review is to give current information of WSSV in Iran, host ranges, carriers, biology, clinical signs, histopathology, PCR, with emphasis on the effects of WSSV in shrimp aquaculture.
    Keywords: Aquaculture ; Biology ; Fisheries ; WSSV ; Shrimp ; Disease ; Aquaculture ; Biology ; Physiology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
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    Format: 1021-1055
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/23622 | 18721 | 2018-07-12 19:36:06 | 23622 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-14
    Description: The pathogenesis of the white spot syndrome virus (WSSV) was studied experimentally with challenge exposure of one hundred post larvae Litopenaeus vannamei each aged 40 days. The shrimp L. vannamei larvae were first examined with Shrimp PC sub(a)R sub(e) Simplex detection kit and found to be free of WSSV. The Fenerropenaeus indicus tissues that showed the clinical signs and were PCR positive for the white spot disease (WSD) were used as the source of WSSV. The challenge exposure was accomplished by feeding minced tissue of the F. indicus to L. vannamei for 24 hours. Based on the results, the L. vannamei revealed the clinical sign of WSD after three days, the PCR was positive and all the shrimp specimens were died after ten days. The control group of the shrimp did not show any sign of WSD and their PCR was negative during the experiment.
    Keywords: Fisheries ; Health ; Pathogenesis ; Whiteleg shrimp ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
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    Format: 1-8
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  • 4
    Publication Date: 2021-07-15
    Description: To introduce Litopenaeus vannamei to Iran as a cultured shrimp species, a experimental study was carried out to assess the effects of different stocking densities on growth rate, mean weight, survival rate, food conversion ratio and the total harvest of L. vannamei. Mee post-larval groups (P1,12) of L. vannamei (pond A: 20 PL/m2, pond B: 30 PL/m2 and pond C: 50 PL/m2) with average size 0.9cm and initial mean weight 0.01±0.005g were used in this study. The experiments were carried out in three replications in 9 earthen ponds each half a hectare in size in Khouzestan province, south of Iran during 110 days. The result showed there was no significant correlation (P〉0.05) between stocking density and survival rate in the semi intensive culture condition. Mean survival rate among farms were greatest at the lowest density, where mean survival in pond A, B, and C was 88.6%, 83.3%, 73.3%, respectively but the differences were not significant (P〉0.05). The results also showed there was no significant relationship (P〉0.05) between growth rate and growth days in each experiment. The mean growth rate/days for ponds A, B, and C were calculated at 0.16g/day, 0.16g/day and 0.15g/day, respectively. The food conversion ratio in the end of the study period for ponds A, B and C was 1.01, 1.17 and 1.2, respectively. Shrimp production increased with an increase in stocking density when farm production at Pond A and B indicated a significant difference (P〈0.05) compared to that of the pond C. The total shrimp production for ponds A, B and C was calculated at 2803kg/ha, 3146kg/ha and 3972kg/ha, respectively. The water temperature ranged between 28±3°C to 23±3°C, with the highest in July and lowest in September. Salinity ranged between 25±2ppt to 12±3ppt, pH ranged between 8.6±0.2 to 7.50.4 and dissolved oxygen ranged between 5.8± 0.4ppm to 5.3±0.3ppm throughout the study. The study proved that L. vannamei could adapt to common conditions in Iranian shrimp culture environment and also we showed that the species production was higher than the native species already under culture.
    Keywords: Biology ; Growth rate ; Weight ; Survival ; Marine ; Stocking density ; Shrimp culture ; Food conversion ; Litopenaeus vannamei ; Kurdistan Province ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
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    Format: 15-22
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25123 | 18721 | 2018-08-26 13:40:27 | 25123 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The WSD is the important viral shrimp disease in past decade. The detection of virus in each country was investigated by polymerase chain reaction for sensitivity and accurate. In this research study we collected 23 samples from shrimp suspected to WSD. The DNA from samples collected and extracted and design two kind of primer from VP24 identified in gene bank by DNAsis software. A primer also designs for Housekeeping gene for positive and negative samples in all examined. The results showed the gene colon for wssv is the similar with others and 97% is consistency. The product of PCR was colon in plasmid and confirmed and this plasmid used for internal control.
    Keywords: Health ; Iran ; White spot syndrome virus ; DNA ; Polymerase chain reaction ; WSD ; Viral ; Shrimp ; Samples ; Penaeus indicus ; Polymerase ; WSSV ; Virus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 39
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  • 6
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25179 | 18721 | 2018-09-03 17:23:17 | 25179 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study two species of algae, Sargassum glaucescens and Padina boergeseni that found plenteously in Persian Gulf and Bushehr coast, were collected and hot water extracts of them were lyophilized. F. indicus (11.32±1.20 g), after two weeks adaptation in Shoghab research station were immersed in seawater (39 ppt and 25±1 ○C) containing hot-water extract of each brown algae, S.glaucescens and P.boergeseni, at 100, 300 and 500 mg/l concentration, Survival rate and immunological parameters (total haemocyte count (THC), total plasma protein (TPP), Phagocytic activity, bacterial clearance efficiency and bactericidal activity) were examined. In addition effect of dietary administration of beta 1, 3 1, 6 glucan on prevention of White Spot Disease and immunological parameters of shrimp were investigated. According to results, immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours or oral administration of beta 1,3 1,6 glucan at level of 10 g/kg diet for 14 days significantly enhanced THC, TPP, Phagocytic activity, bacterial clearance efficiency and bactericidal activity. Immersion in seawater containing 100, 300 and 500 mg/l hot-water extract of S.glaucescens after 3 hours, improved the survival rate of WSSV-infected F. indicus.
    Keywords: Biology ; Chemistry ; Iran ; Persian Gulf ; Bushehr Province ; Beta-glucan ; Sargassum ; Padina algae ; WSD ; Fenneropenaeus indicus ; Species ; Algae ; Brown Algae ; S.glaucescens ; P.boergeseni ; Survival rate ; White Spot Disease ; Shrimp
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 57
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  • 7
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25552 | 18721 | 2018-10-05 17:30:01 | 25552 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Biosecurity means” all activities that decrease the introducing and expanding the pathogen in the shrimp farm” the main activity consisit physical, chemical and biological methods. Some factores has low risck and some of them have high risck level. The level of biosecurity in the shrimp farm and hatcheries is depends the equipmemt and expense that used in the program. For each situation in biosecurity, the scientist will be prepared the standard operation program (SOP) for each risck factor.In the Specific Pathogen Free program that conduted in Bousher, 10 SOP design for different risck factor in the banderga station and Persian Golf Centre for SPF production. The SOP, detecting the main pathogen in shrimp farm in shrimp, feed, water and any equipment in the project. The SOP also consider the disinfectant water with physical and chemival methods such as different net in different size, chemical such as chlorine and ozon and biological filter. During the project studied the methods exclude all pathogen from shrimp and feed and decreased the risck factors in program. The result showed if the culturist used these methods in the production, the safty of shrimp production increased and the sustainable shrimp culture will be available.
    Keywords: Health ; Iran ; Biosecurity ; Ciritical point ; Standard Operation Porogram ; Disaese ; SOP ; Implementation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 65
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  • 8
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25615 | 18721 | 2018-10-07 16:19:08 | 25615 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Aras dam reservoir situated in the northwest of Iran, west Azarbaijan province, is the only water resource of Astacus leptodactylus harvest in the country that more than 250tons of this species were exported to different countries all over the world, annually. On the other hand, one of the polices of Iranian Science Fisheries Institute is the release of this species into other water resources in the country and for this purpose, the study of risky diseases such as Crayfish pest (Aphanomysis astasi) and other zoonotic diseases are considered as the research priorities of aquaculture development of the country. This study was carried out to health screening of Astacus leptodactylus at Aras dam reservoir from winter 2013 to fall 2014. In this regard, A total of 394 harvested livefreshwater crayfish Astacus leptodactylus (255males, 139females) weretested. 9 epibionts and parasites peritrich protozoans were identified. From Metazoan parasites group, Branchiobdella kozarovi with incidence rate of (100%) in obtained samples was the only isolated organism from this group that identified up to species level. There was a heavy damage in gills of samples with Aeolosoma hemprichi (Annelid) in winter with90% prevalence. Furthermore, Other Epibiont fouling organisms such as Rotatoria; free living nematods and suctoria were observed in this survey. The fungi study of the lesions and melanized spots of mentioned samples revealed their infection to Penicillium expansum; Aspergillus flavus; Alternaria sp. ; Fusarium sp. and Saprolegnia sp. The results of bacterial study confirmed the presence of pathogen bacteria in Astacus leptodactylus. The most frequency percentage (15.16%) in hepatopancrease were related to Aeromonas hydrophila and the least one (1.37%) were due to Yersinia bacteria. Also, only Aeromonas hydrophila and Staphylococcus aureus were isolated and identified from heamolymph, respectively. The results revealed that the combination of Salmonella typhi, Escherichia coli and Staphylococcus sp. has caused the most infection rate while. Yersinia ruckeri and Salmonella typhi has caused the least infections in Astacus leptodactylus. According to the isolation of 6 bacteria species from hepatopancreas and 2 species from heamplymph , it can be concluded that hepatopancreas enjoyed the higher infection rate compared to haemolymph in the obtained samples.
    Keywords: Ecology ; Iran ; Aras Dam Reservoir ; West Azarbaijan province ; Astacus leptodactylus ; Health screening ; Hygienic ; Freshwater ; Crayfish ; Species ; Fisheries ; Aphanomysis astasi ; Parasites ; Metazoa ; Aeolosoma hemprichi ; Epibiont fouling ; Alternaria sp.
    Repository Name: AquaDocs
    Type: monograph
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  • 9
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25807 | 18721 | 2018-10-13 10:13:41 | 25807 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: White spot disease (WSD) is one of the shrimp deadliest viral diseases that causes heavy losses on all shrimp of Penaeid family. Most invertebrates are lacking adaptive immune system and its defense is the innate immune system that is as cellular and humoral, but a like immune system against white spot virus in shrimp was been detected. In this research, control and prevention of white spot disease in shrimp using algae Gracilaria corticata, investigated. About 300 vannamei shrimp were divided to 4 groups and fed by normal pellet and algae extract in 14 days. At the end of the fourteenth day half of the shrimp were challenged with acute white spot virus. After the fourteenth day in the days 0, 3, 9, 18 and 25 sampling were done from the hemolymph of survived shrimps and survival and immune factors were evaluated. Based on results, in the challenge test, shrimps that fed with algae extract has a significant survival rate than shrimp fed with commercial diet. Increased the Immune Factors from day one to day 25 observed during the test. Greatest amount of Immune Factors THC, TPP, SOD, POD and PO in T1 group were observed in day 25 of tests. This situation was also true for group2 (T2), but its rate significantly was less than group 1(T1).
    Keywords: Aquaculture ; Health ; Iran ; Evaluation ; Immunity factors ; THC ; TPP ; PO ; SOD ; POD ; Shrimp ; Algae ; Gracilaria corticata ; White spot virus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 62
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  • 10
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    In:  http://aquaticcommons.org/id/eprint/21811 | 18721 | 2018-01-08 09:35:59 | 21811 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-02
    Description: Presence of hepatopancreatic parvo-like vines (HPV) disease was assessed from June until October 2009 in Litopenaeus vannamei hatcheries and grow-out farms of the Bushehr province. Samples were collected from 6 hatcheries and 6 grow-out farms located in coasted areas. From each hatchery, 100 PL samples with average age PL5-PL8 and 20-30 samples from each grow-out farm with average age 105 to 120 days were collected. The samples were divided into three groups one used for gross sign and wet mount with Gimsa, the second group was preserved in Davidson Fixative and used for histopathology and the third group was fixed in ethyl alcohol 95% and used for polymerase chain Reaction (PCR). In gross sign 30%- 40% of the shrimp showed different sizes and some were smaller than the others. In the wet mount group with Gimsa staining of hepatopancrease, the inclusion body with basophilic color was seen. The histopathology indicated that the hepatopancreatic cell was infected and the basophilic inclusion body observed in many samples. The PCR examined with IQ 2000 Kit was negative. The rate of infection (ROI) was 1.1% for hatcheries and 32% for grow-out farms.
    Keywords: Aquaculture ; Biology ; Litopenaveus vannamei ; HPV ; Gross ; Sign ; Histopathology ; PCR ; Iran ; Bushehr Province
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 107-118
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