ISSN:
1432-2145
Keywords:
Microfilament
;
Microtubule
;
Pollen tube growth
;
Propiconazole
;
Tradescantia virginiana
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The effects of propiconazole on germination and tube growth of Tradescantia virginiana pollen when incorporated in germination media at 0, 102, 136, or 170 μl l−1 were evaluated using light microscopy and immunocytochemistry. Propiconazole inhibited pollen germination, cytoplasmic streaming, and tube elongation. Treatments also induced abnormal tube morphology and cytoskeletal distribution. Tubes treated with propiconazole displayed weaker microfilament (Mf) signals along the pollen tubes, with amorphous staining. Microtubule (Mt) distribution was also severely affected. In treated tubes, the proximal portions had characteristically fragmented Mts. Fewer Mt bundles were seen in the subapical region, and these were located further from the apex. Propiconazole effects were generally concentration dependent. The results indicate that propiconazole affects both Mfs and Mts; however, the effects may be an indirect result of the drug's influence on membranes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00228939
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