ALBERT

Description: A reasonable plant type is an essential factor for improving canopy structure, ensuring a reasonable expansion of the leaf area index and obtaining a high-quality spatial distribution of light. It is of great significance in promoting effective selection of the ecological breeding index and production practices for maize. In this study, a method for calculating the phenotypic traits of the maize canopy in three-dimensional (3D) space was proposed, focusing on the problems existing in traditional measurement methods in maize morphological structure research, such as their complex procedures and relatively large error margins. Specifically, the whole maize plant was first scanned with a FastSCAN hand-held scanner to obtain 3D point cloud data for maize. Subsequently, the raw point clouds were simplified by the grid method, and the effect of noise on the quality of the point clouds in maize canopies was further denoised by bilateral filtering. In the last step, the 3D structure of the maize canopy was reconstructed. In accordance with the 3D reconstruction of the maize canopy, the phenotypic traits of the maize canopy, such as plant height, stem diameter and canopy breadth, were calculated by means of a fitting sphere and a fitting cylinder. Thereafter, multiple regression analysis was carried out, focusing on the calculated data and the actual measured data to verify the accuracy of the calculation method proposed in this study. The corresponding results showed that the calculated values of plant height, stem diameter and plant width based on 3D scanning were highly correlated with the actual measured data, and the determinant coefficients R2 were 0.9807, 0.8907 and 0.9562, respectively. In summary, the method proposed in this study can accurately measure the phenotypic traits of maize. Significantly, these research findings provide technical support for further research on the phenotypic traits of other crops and on variety breeding.

Description: Volatile organic compounds (VOCs) released by plants are closely associated with plant metabolism and can serve as biomarkers for disease diagnosis. Huanglongbing (HLB), also known as citrus greening or yellow shoot disease, is a lethal threat to the multi-billion-dollar citrus industry. Early detection of HLB is vital for removal of susceptible citrus trees and containment of the disease. Gas sensors are applied to monitor the air quality or toxic gases owing to their low-cost fabrication, smooth operation, and possible miniaturization. Here, we report on the development, characterization, and application of electrical biosensor arrays based on single-walled carbon nanotubes (SWNTs) decorated with single-stranded DNA (ssDNA) for the detection of four VOCs—ethylhexanol, linalool, tetradecene, and phenylacetaldehyde—that serve as secondary biomarkers for detection of infected citrus trees during the asymptomatic stage. SWNTs were noncovalently functionalized with ssDNA using π–π interaction between the nucleotide and sidewall of SWNTs. The resulting ssDNA-SWNT hybrid structure and device properties were investigated using Raman spectroscopy, ultraviolet (UV) spectroscopy, and electrical measurements. To monitor changes in the four VOCs, gas biosensor arrays consisting of bare SWNTs before and after being decorated with different ssDNA were employed to determine the different concentrations of the four VOCs. The data was processed using principal component analysis (PCA) and neural net fitting (NNF).

Description: Short-term characteristics of urban land cover change have been observed and reported from satellite images, although urban landscapes are mainly influenced by anthropogenic factors. These short-term changes in urban areas are caused by rapid urbanization, seasonal climate changes, and phenological ecological changes. Quantifying and understanding these short-term characteristics of changes in various land cover types is important for numerous urban studies, such as urbanization assessments and management. Many previous studies mainly investigated one study area with insufficient datasets. To more reliably and confidently investigate temporal variation patterns, this study employed Fourier series to quantify the seasonal changes in different urban land cover types using all available Landsat images over four different cities, Melbourne, Sao Paulo, Hamburg, and Chicago, within a five-year period (2011–2015). The overall accuracy was greater than 86% and the kappa coefficient was greater than 0.80. The R-squared value was greater than 0.80 and the root mean square error was less than 7.2% for each city. The results indicated that (1) the changing periods for water classes were generally from half a year to one and a half years in different areas; and, (2) urban impervious surfaces changed over periods of approximately 700 days in Melbourne, Sao Paulo, and Hamburg, and a period of approximately 215 days in Chicago, which was actually caused by the unavoidable misclassification from confusions between various land cover types using satellite data. Finally, the uncertainties of these quantification results were analyzed and discussed. These short-term characteristics provided important information for the monitoring and assessment of urban areas using satellite remote sensing technology.