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  • 1
    Keywords: Ecology . ; Water. ; Hydrology. ; Environmental chemistry. ; Environmental sciences. ; Physics. ; Environment. ; Ecology. ; Water. ; Environmental Chemistry. ; Environmental Physics. ; Environmental Sciences.
    Description / Table of Contents: Chapter 1 Measurement of environmental parameters affecting marine plankton physiology -- Chapter 2 Plankton culture techniques -- Chapter 3 Determination of Key Enzymes involved in primary productivity -- Chapter 4 Analyses of pigments -- Chapter 5 Measurements and analyses of photosynthesis and respiration -- Chapter 6 Chlorophyll fluorescence techniques and applications -- Chapter 7 Biochemical and molecular methods -- Chapter 8 Research methods for animal and virus.
    Abstract: This book presents methods for investigating the effects of aquatic environmental changes on organisms and the mechanisms involved. It focuses mainly on photosynthetic organisms, but also provides methods for virus, zooplankton and other animal studies. Also including a comprehensive overview of the current methods in the fields of aquatic physiology, ecology, biochemistry and molecular approaches, including the advantages and disadvantages of each method, the book is a valuable guide for young researchers in marine or aquatic sciences studying the physiological processes associated with chemical and physical environmental changes.
    Type of Medium: Online Resource
    Pages: XI, 352 p. 83 illus., 23 illus. in color. , online resource.
    Edition: 1st ed. 2021.
    ISBN: 9789811553547
    DDC: 577
    Language: English
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  • 2
    Call number: 9789811553547 (e-book)
    Description / Table of Contents: This book presents methods for investigating the effects of aquatic environmental changes on organisms and the mechanisms involved. It focuses mainly on photosynthetic organisms, but also provides methods for virus, zooplankton and other animal studies. Also including a comprehensive overview of the current methods in the fields of aquatic physiology, ecology, biochemistry and molecular approaches, including the advantages and disadvantages of each method, the book is a valuable guide for young researchers in marine or aquatic sciences studying the physiological processes associated with chemical and physical environmental changes.
    Type of Medium: 12
    Pages: 1 Online-Ressource (XI, 352 Seiten) , Illustrationen
    ISBN: 9789811553547 , 978-981-15-5354-7
    Language: English
    Note: Contents Part I Measurement of Environmental Parameters Affecting Marine Plankton Physiology 1 Characteristics of Marine Chemical Environment and the Measurements and Analyses of Seawater Carbonate Chemistry / Weidong Zhai 2 Photosynthetically Active Radiation and Ultraviolet Radiation Measurements / Gang Li and Kunshan Gao Part II Plankton Culture Techniques 3 Manipulation of Seawater Carbonate Chemistry / Kunshan Gao 4 Microalgae Continuous and Semi-continuous Cultures / Shanwen Chen and Kunshan Gao 5 Culturing Techniques for Planktonic Copepods / Wei Li, Xin Liu, and Zengling Ma Part III Determination of Key Enzymes in Primary Producers 6 Carbonic Anhydrase / Jianrong Xia, Xiongwen Chen, and Mario Giordano 7 Rubisco / Cuimin Liu, Kaiyao Huang, and Jianrong Xia 8 Phosphoenolpyruvate Carboxylase / Fan Hu and Hanhua Hu 9 Nitrate Reductase / Dinghui Zou 10 Antioxidants and Reactive Oxygen Species (ROS) Scavenging Enzymes / Yahe Li and Zengling Ma Part IV Measurements and Analyses of Pigments 11 Chlorophylls / Wenting Ke, Yanchao Yin, Xiongwen Chen, and Baosheng Qiu 12 Phycobiliproteins / Yiwen Yang, Juntian Xu, and Baosheng Qiu 13 Carotenoids / Fan Hu and Hanhua Hu 14 Phenolic Compounds and Other UV-Absorbing Compounds / Peng Jin and Kunshan Gao Part V Measurements and Analyses of Photosynthesis and Respiration 15 Photosynthetic Oxygen Evolution / Guozheng Dai, Hualing Mi, and Baosheng Qiu 16 Photosynthetic Carbon Fixation / Gang Li, Yaping Wu, and Guang Gao 17 Photorespiration and Dark Respiration / Dinghui Zou and Juntian Xu 18 Carbon Dioxide vs. Bicarbonate Utilisation / Sven Beer, Mats Björk, and John Beardall 19 Action Spectra of Photosynthetic Carbon Fixation / Yaping Wu, Gang Li, and Kunshan Gao 20 Determination of the Inorganic Carbon Affinity and CO2 Concentrating Mechanisms of Algae / Yaping Wu and Kunshan Gao 21 Methods for Measuring Algal Carbon Fixation in Flow-Through Seawater / Kunshan Gao and Juntian Xu 22 Application of Membrane-Inlet Mass Spectrometry to Measurements of Photosynthetic Processes / Kunshan Gao and Hualing Mi 23 SIMS and NanoSIMS Techniques Applied to Studies of Plankton Productivity / Helle Ploug 24 Measurements of Photoinactivation and Repair of Photosystem II / Gang Li, Yahe Li, Wanchun Guan, and Hongyan Wu Part VI Chlorophyll Fluorescence Techniques and Applications 25 Basic Concepts and Key Parameters of Chlorophyll Fluorescence / Sven Beer, Mats Björk, and John Beardall 26 Fluorescence Measurement Techniques / Sven Beer, Mats Björk, and John Beardall 27 Carbon Assimilation Capacity and Blue-Green Fluorescence / Hualing Mi and Baosheng Qiu 28 In Situ Measurement of Phytoplankton Photochemical Parameters / Guang Gao, Peng Jin, and Kunshan Gao Part VII Biochemical and Molecular Methods 29 Biochemical Inhibitors for Algae / Yaping Wu and Kunshan Gao 30 Measurements of Particulate Organic Carbon, Nitrogen, and Phosphorus / Kai Xu, Kunshan Gao, Fei-xue Fu, and David A. Hutchins 31 Isolation of Organelles / Min Xu and Hualing Mi 32 Measurements of Calcification and Silicification / Kai Xu, Kunshan Gao, and David A. Hutchins 33 Use of the Fluorochrome Calcein to Measure Growth and Calcification in Marine Organisms / Sam Dupont 34 The Application of Transcriptomics, Metagenomics, and Metatranscriptomics in Algal Research / Xin Lin 35 Methods for Nitrogen Fixation Measurement / Feixue Fu and Pingping Qu 36 Trace Metal Clean Culture Techniques / Yuanyuan Feng, Feixue Fu, and David A. Hutchins Part VIII Research Methods for Animals and Viruses 37 Electrophysiological Recording in Fish / Xiaojie Wang 38 Heart Rate Measurement in Mollusks / Yunwei Dong, Guodong Han, and Xiaoxu Li 39 Measuring the Feeding Rate of Herbivorous Zooplankton / Wei Li and Zengling Ma 40 Measurement of Virus-Induced Phytoplankton Mortality / Dapeng Xu, Yunlan Yang, and Rui Zhang
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  • 3
    Publication Date: 2023-02-24
    Keywords: Acoustic Doppler Current Profiler; ADCP; Current velocity, east-west; Current velocity, north-south; DATE/TIME; DEPTH, water; LATITUDE; LONGITUDE; Point Sur; PS99A6; PS99A6_00451; Shipboard Acoustic Doppler Current Profiling (SADCP); Ship velocity, absolute east-west, standard deviation; Ship velocity, absolute east-west components means; Ship velocity, absolute north-south components mean; Ship velocity, absolute north-south standard deviation; Temperature, technical; Temperature, technical, standard deviation; WOCE; World Ocean Circulation Experiment
    Type: Dataset
    Format: text/tab-separated-values, 8236 data points
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  • 4
    Publication Date: 2024-03-14
    Keywords: Calcium carbonate production of carbon; Calcium carbonate production of carbon, standard deviation; Coccolithophoridae, total; Cruise/expedition; DATE/TIME; DEPTH, water; Emiliania huxleyi; Incubation duration; LATITUDE; LONGITUDE; Method comment; Ocean and sea region; Percentage; Primary production of carbon; Primary production of carbon, standard deviation; Principal investigator; Reference/source; Station label; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 35037 data points
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  • 5
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    In:  Supplement to: Gao, Kunshan; Xu, Juntian; Gao, Guang; Li, Yahe; Hutchins, David A; Huang, Bangqin; Wang, Lei; Zheng, Ying; Jin, Peng; Cai, Xiaoni; Häder, Donat-Peter; Li, Wei; Xu, Kai; Liu, Nana; Riebesell, Ulf (2012): Rising CO2 and increased light exposure synergistically reduce marine primary productivity. Nature Climate Change, 2, 519–523, https://doi.org/10.1038/nclimate1507
    Publication Date: 2024-03-15
    Description: Carbon dioxide and light are two major prerequisites of photosynthesis. Rising CO2 levels in oceanic surface waters in combination with ample light supply are therefore often considered stimulatory to marine primary production. Here we show that the combination of an increase in both CO2 and light exposure negatively impacts photosynthesis and growth of marine primary producers. When exposed to CO2 concentrations projected for the end of this century, natural phytoplankton assemblages of the South China Sea responded with decreased primary production and increased light stress at light intensities representative of the upper surface layer. The phytoplankton community shifted away from diatoms, the dominant phytoplankton group during our field campaigns. To examine the underlying mechanisms of the observed responses, we grew diatoms at different CO2 concentrations and under varying levels (5-100%) of solar radiation experienced by the phytoplankton at different depths of the euphotic zone. Above 22-36% of incident surface irradiance, growth rates in the high-CO2-grown cells were inversely related to light levels and exhibited reduced thresholds at which light becomes inhibitory. Future shoaling of upper-mixed-layer depths will expose phytoplankton to increased mean light intensities. In combination with rising CO2 levels, this may cause a widespread decline in marine primary production and a community shift away from diatoms, the main algal group that supports higher trophic levels and carbon export in the ocean.
    Keywords: A4_SCS; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (〈20 L); C3_SCS; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Chlorophyll a; Chromista; Coast and continental shelf; DATE/TIME; Duration; E606_SCS; East China Sea; Entire community; Event label; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; In situ sampler; Irradiance; Irradiance, standard deviation; ISS; Laboratory experiment; LE04_SCS; Light; Non photochemical quenching; Non photochemical quenching, standard deviation; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Phaeodactylum tricornutum; Phosphate; Phytoplankton; PN07_ECS; Potentiometric; Primary production/Photosynthesis; Primary production of carbon; Primary production of carbon, per chlorophyll a; Primary production of carbon, per volume of seawater; Primary production of carbon, standard deviation; Salinity; Season; SEATS_SCS; Single species; Skeletonema costatum; South China Sea; Species; Temperate; Temperature, water; Thalassiosira pseudonana; Time of day; Treatment; Tropical; Yield ratio
    Type: Dataset
    Format: text/tab-separated-values, 17109 data points
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  • 6
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    In:  Supplement to: Tong, Shanying; Hutchins, David A; Gao, Kunshan (2019): Physiological and biochemical responses of Emiliania huxleyi to ocean acidification and warming are modulated by UV radiation. Biogeosciences, 16(2), 561-572, https://doi.org/10.5194/bg-16-561-2019
    Publication Date: 2024-03-15
    Description: Marine phytoplankton such as bloom-forming, calcite-producing coccolithophores, are naturally exposed to solar ultraviolet radiation (UVR, 280–400 nm) in the ocean's upper mixed layers. Nevertheless, the effects of increasing carbon dioxide (CO2)-induced ocean acidification and warming have rarely been investigated in the presence of UVR. We examined calcification and photosynthetic carbon fixation performance in the most cosmopolitan coccolithophorid, Emiliania huxleyi, grown under high (1000 µatm, HC; pHT: 7.70) and low (400 µatm, LC; pHT: 8.02) CO2 levels, at 15 °C, 20 °C and 24 °C with or without UVR. The HC treatment did not affect photosynthetic carbon fixation at 15 ∘C, but significantly enhanced it with increasing temperature. Exposure to UVR inhibited photosynthesis, with higher inhibition by UVA (320–395 nm) than UVB (295–320 nm), except in the HC and 24 °C-grown cells, in which UVB caused more inhibition than UVA. A reduced thickness of the coccolith layer in the HC-grown cells appeared to be responsible for the UV-induced inhibition, and an increased repair rate of UVA-derived damage in the HC–high-temperature grown cells could be responsible for lowered UVA-induced inhibition. While calcification was reduced with elevated CO2 concentration, exposure to UVB or UVA affected the process differentially, with the former inhibiting it and the latter enhancing it. UVA-induced stimulation of calcification was higher in the HC-grown cells at 15 and 20 °C, whereas at 24 °C observed enhancement was not significant. The calcification to photosynthesis ratio (Cal ∕ Pho ratio) was lower in the HC treatment, and increasing temperature also lowered the value. However, at 20 and 24 °C, exposure to UVR significantly increased the Cal ∕ Pho ratio, especially in HC-grown cells, by up to 100 %. This implies that UVR can counteract the negative effects of the “greenhouse” treatment on the Cal ∕ Pho ratio; hence, UVR may be a key stressor when considering the impacts of future greenhouse conditions on E. huxleyi.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcification/Dissolution; Calcification rate of carbon per cell; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, inorganic, particulate, per cell; Carbon, organic, particulate, per cell; Carbon/Nitrogen ratio; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Chromista; Emiliania huxleyi; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Haptophyta; Laboratory experiment; Laboratory strains; Light; Nitrogen, organic, particulate, per cell; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate inorganic carbon/particulate organic carbon ratio; Pelagos; pH; pH, standard deviation; Photosynthetic carbon fixation rate, per cell; Phytoplankton; Primary production/Photosynthesis; Registration number of species; Replicate; Salinity; Single species; Temperature; Temperature, water; Treatment; Uniform resource locator/link to reference; Volume
    Type: Dataset
    Format: text/tab-separated-values, 2250 data points
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  • 7
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    In:  Supplement to: Liu, Nana; Tong, Shanying; Yi, Xiangqi; Li, Yan; Li, Zhenzhen; Miao, Hangbin; Wang, Tifeng; Li, Futian; Yan, Dong; Huang, Ruiping; Wu, YaPing; Hutchins, David A; Beardall, John; Dai, Minhan; Gao, Kunshan (2017): Carbon assimilation and losses during an ocean acidification mesocosm experiment, with special reference to algal blooms. Marine Environmental Research, 129, 229-235, https://doi.org/10.1016/j.marenvres.2017.05.003
    Publication Date: 2024-03-15
    Description: A mesocosm experiment was conducted in Wuyuan Bay (Xiamen), China, to investigate the effects of elevated pCO2 on bloom formation by phytoplankton species previously studied in laboratory-based ocean acidification experiments, to determine if the indoor-grown species performed similarly in mesocosms under more realistic environmental conditions. We measured biomass, primary productivity and particulate organic carbon (POC) as well as particulate organic nitrogen (PON). Phaeodactylum tricornutum outcompeted Thalassiosira weissflogii and Emiliania huxleyi, comprising more than 99% of the final biomass. Mainly through a capacity to tolerate nutrient-limited situations, P. tricornutum showed a powerful sustained presence during the plateau phase of growth. Significant differences between high and low CO2 treatments were found in cell concentration, cumulative primary productivity and POC in the plateau phase but not during the exponential phase of growth. Compared to the low pCO2 (LC) treatment, POC increased by 45.8–101.9% in the high pCO2 (HC) treated cells during the bloom period. Furthermore, respiratory carbon losses of gross primary productivity were found to comprise 39–64% for the LC and 31–41% for the HC mesocosms (daytime C fixation) in phase II. Our results suggest that the duration and characteristics of a diatom bloom can be affected by elevated pCO2. Effects of elevated pCO2 observed in the laboratory cannot be reliably extrapolated to large scale mesocosms with multiple influencing factors, especially during intense algal blooms.
    Keywords: Alkalinity, total; Aragonite saturation state; Bicarbonate ion; Biomass/Abundance/Elemental composition; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, organic, particulate; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cell density; Chlorophyll a; Coast and continental shelf; Community composition and diversity; Day of experiment; Entire community; EXP; Experiment; Field experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Mesocosm or benthocosm; Nitrogen, organic, particulate; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Phosphate; Primary production/Photosynthesis; Primary production of carbon per day; Registration number of species; Replicate; Respiration rate, carbon dioxide; Salinity; Silicate; Species; Temperate; Temperature, water; Treatment; Type; Uniform resource locator/link to reference; Wuyuan_Bay
    Type: Dataset
    Format: text/tab-separated-values, 12180 data points
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  • 8
    Publication Date: 2024-03-15
    Description: Coccolithophores are important oceanic primary producers not only in terms of photosynthesis but also because they produce calcite plates called coccoliths. Ongoing ocean acidification associated with changing seawater carbonate chemistry may impair calcification and other metabolic functions in coccolithophores. While short‐term ocean acidification effects on calcification and other properties have been examined in a variety of coccolithophore species, long‐term adaptive responses have scarcely been documented, other than for the single species Emiliania huxleyi . Here, we investigated the effects of ocean acidification on another ecologically important coccolithophore species, Gephyrocapsa oceanica, following 1,000 generations of growth under elevated CO2 conditions (1,000 μatm). High CO2‐selected populations exhibited reduced growth rates and enhanced particulate organic carbon (POC ) and nitrogen (PON ) production, relative to populations selected under ambient CO2 (400 μatm). Particulate inorganic carbon (PIC ) and PIC /POC ratios decreased progressively throughout the selection period in high CO2‐selected cell lines. All of these trait changes persisted when high CO2‐grown populations were moved back to ambient CO2 conditions for about 10 generations. The results suggest that the calcification of some coccolithophores may be more heavily impaired by ocean acidification than previously predicted based on short‐term studies, with potentially large implications for the ocean's carbon cycle under accelerating anthropogenic influences.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, inorganic, particulate, per cell; Carbon, organic, particulate, per cell; Carbon/Nitrogen ratio; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Chromista; Day of experiment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gephyrocapsa oceanica; Growth/Morphology; Growth rate; Haptophyta; Laboratory experiment; Laboratory strains; Nitrogen, organic, particulate, per cell; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate inorganic carbon/particulate organic carbon ratio; pH; pH, standard deviation; Phytoplankton; Potentiometric; Registration number of species; Replicate; Salinity; Single species; Species; Temperature, water; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 12720 data points
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  • 9
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    In:  Supplement to: Li, Futian; Wu, YaPing; Hutchins, David A; Fu, Feixue; Gao, Kunshan (2016): Physiological responses of coastal and oceanic diatoms to diurnal fluctuations in seawater carbonate chemistry under two CO2 concentrations. Biogeosciences, 13(22), 6247-6259, https://doi.org/10.5194/bg-13-6247-2016
    Publication Date: 2024-04-03
    Description: Diel and seasonal fluctuations in seawater carbonate chemistry are common in coastal waters, while in the open-ocean carbonate chemistry is much less variable. In both of these environments, ongoing ocean acidification is being superimposed on the natural dynamics of the carbonate buffer system to influence the physiology of phytoplankton. Here, we show that a coastal Thalassiosira weissflogii isolate and an oceanic diatom, Thalassiosira oceanica, respond differentially to diurnal fluctuating carbonate chemistry in current and ocean acidification (OA) scenarios. A fluctuating carbonate chemistry regime showed positive or negligible effects on physiological performance of the coastal species. In contrast, the oceanic species was significantly negatively affected. The fluctuating regime reduced photosynthetic oxygen evolution rates and enhanced dark respiration rates of T. oceanica under ambient CO2 concentration, while in the OA scenario the fluctuating regime depressed its growth rate, chlorophyll a content, and elemental production rates. These contrasting physiological performances of coastal and oceanic diatoms indicate that they differ in the ability to cope with dynamic pCO2. We propose that, in addition to the ability to cope with light, nutrient, and predation pressure, the ability to acclimate to dynamic carbonate chemistry may act as one determinant of the spatial distribution of diatom species. Habitat-relevant diurnal changes in seawater carbonate chemistry can interact with OA to differentially affect diatoms in coastal and pelagic waters.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Biogenic particulate silica/Carbon, organic, particulate; Biogenic particulate silica/Carbon, organic, particulate, standard deviation; Biogenic silica, per cell; Biogenic silica, standard deviation; Biogenic silica production, standard deviation; Biogenic silica production per cell; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Cell size; Cell size, standard deviation; Chlorophyll a, production, standard deviation; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chlorophyll a production per cell; Chromista; Effective photochemical quantum yield; Effective photochemical quantum yield, standard deviation; Figure; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Laboratory experiment; Laboratory strains; Net photosynthesis rate, oxygen, per cell; Net photosynthesis rate, oxygen, per chlorophyll a; Net photosynthesis rate, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Other; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon, per cell; Particulate organic carbon, production, standard deviation; Particulate organic carbon content per cell, standard deviation; Particulate organic carbon production per cell; Particulate organic nitrogen, standard deviation; Particulate organic nitrogen per cell; Particulate organic nitrogen production, standard deviation; Pelagos; pH; pH, standard deviation; Phytoplankton; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Production of particulate organic nitrogen; Registration number of species; Respiration; Respiration/net photosynthesis ratio; Respiration/net photosynthesis ratio, standard deviation; Respiration rate, oxygen, per cell; Respiration rate, oxygen, standard deviation; Salinity; Single species; Species; Table; Temperature, water; Thalassiosira oceanica; Thalassiosira weissflogii; Time in hours; Treatment; Type; Uniform resource locator/link to reference
    Type: Dataset
    Format: text/tab-separated-values, 1944 data points
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  • 10
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    In:  Supplement to: Gao, Guang; Jin, Peng; Liu, Nana; Li, Futian; Tong, Shanying; Hutchins, David A; Gao, Kunshan (2017): The acclimation process of phytoplankton biomass, carbon fixation and respiration to the combined effects of elevated temperature and p CO 2 in the northern South China Sea. Marine Pollution Bulletin, 118(1-2), 213-220, https://doi.org/10.1016/j.marpolbul.2017.02.063
    Publication Date: 2024-03-15
    Description: We conducted shipboard microcosm experiments at both off-shore (SEATS) and near-shore (D001) stations in the northern South China Sea (NSCS) under three treatments, low temperature and low pCO2 (LTLC), high temperature and low pCO2 (HTLC), and high temperature and high pCO2 (HTHC). Biomass of phytoplankton at both stations were enhanced by HT. HTHC did not affect phytoplankton biomass at station D001 but decreased it at station SEATS. HT alone increased net primary productivity by 234% at station SEATS and by 67% at station D001 but the stimulating effect disappeared when HC was combined. HT also increased respiration rate by 236% at station SEATS and by 87% at station D001 whereas HTHC reduced it by 61% at station SEATS and did not affect it at station D001. Overall, our findings indicate that the positive effect of ocean warming on phytoplankton assemblages in NSCS could be damped or offset by ocean acidification.
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Chlorophyll a; Chlorophyll a, standard deviation; Coast and continental shelf; Containers and aquaria (20-1000 L or 〈 1 m**2); D001; Entire community; Event label; EXP; Experiment; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Open ocean; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Potentiometric; Primary production/Photosynthesis; Primary production of carbon; Primary production of carbon, standard deviation; Primary production of carbon per chlorophyll a; Respiration; Respiration/net photosynthesis ratio; Respiration/net photosynthesis ratio, standard deviation; Respiration rate, carbon; Respiration rate, carbon, per chlorophyll a; Respiration rate, carbon dioxide, standard deviation; Salinity; SEATS; Station label; Temperature; Temperature, water; Temperature, water, standard deviation; Treatment; Tropical; Type
    Type: Dataset
    Format: text/tab-separated-values, 316 data points
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