ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The influence of inoculum size on the growth kinetics of Clostridium botulinum 56A and percentage of growth-positive samples was studied in a complete factorial design with factors of inoculum size (1, 100, or 10,000 spores), pH, and sodium-chloride concentration. Growth was followed hourly as change in A620. Polynomial regression was used to analyze the data. The time-to-detection and percent growth-positive samples were significantly affected by inoculum size and its quadratic term. When inoculum size increased from 1 to 100 spores/sample, the percent growth-positive samples increased, and the time-to-detection decreased. When the inoculum was 1000 spores/sample or higher, there was little additional effect. Inoculum size might influence results through simple probability or quorum sensing. The maximum growth rate was independent of inoculum levels.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.2000.tb10614.x
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