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  • 1
    ISSN: 1662-0356
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Natural Sciences in General , Technology
    Notes: (Na0.5Bi0.5)TiO3 (Abbreviated to BNT) was synthesized by hydrothermal process and theaverage gain size was 15 nm with narrow size distribution. Pure perovskite structure of BNT particleswas identified by XRD analysis. The nanoscale BNT powder was selected as raw material to fabricateBNT based ceramics with BaTiO3 (Abbreviated to BT) and Ba(Cu0.5W0.5)O3 (Abbreviated to BCW)doping. SEM images show that the ceramic using nanoscale BNT powder as raw material has a highdense body with the average gain size from 0.5 μm to 1 μm. The experimental results show that withincreasing amount of BCW, d33 and Kp of (0.94-x)BNT-0.06BT-xBCW increase initially, then reachthe maximum values of 130 pC/N and 0.32 when 5mol% of BCW was added. Further increasingamount of BCW leads to decreasing d33 and Kp. The optimal piezoelectric and dielectric propertieswere obtained as d33 = 130 pC/N, Kp = 0.32, εr = 800, tgδ = 0.06
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 336-338 (Apr. 2007), p. 7-9 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The influences of the substitution of (Zn1/3Nb2/3) group for Ti as well as addition of Fe2O3 andK2CO3 on d33, kp, εr, and tgδ of the Pb[Zr0.46Ti0.54-x(Zn1/3Nb2/3)x]O3 + y mol%Fe2O3+z mol%K2CO3 (PZNPZT)(0 ≤ x ≤ 0.20, 0 ≤ y ≤ 0.10, 0 ≤ z ≤ 0.20) ceramics were investigated. The experimental resultsdemonstrated that when x increases in Pb[Zr0.46Ti0.54-x(Zn1/3Nb2/3)x]O3+0.3 mol%Fe2O3+0.5mol%K2CO3ceramics, the d33, kp, εr, and tgδ increase and approach to their maximum values, then eventually decrease.XRD analysis revealed that the perovskite structure of the materials transfers from tetragonal phase torhombohedral phase. The relative dielectric constant (εr) decreases dramatically in the rhombohedralphase area near the MPB; the d33 and εr decrease with the small amount of added Fe2O3 and K2CO3, whichalso contributes to densification of the PZN-PZT ceramics. The optimized piezoelectric performancesthrough the experiment were obtained as d33=280 pC/N, kp=0.62, εr=900, tgδ=0.003
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 336-338 (Apr. 2007), p. 1038-1041 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The dispersion and stability of PZT aqueous suspensions were investigated by usingammonium salt of polyacrylic acid (PMAA-NH4) as the dispersant. By observing the sedimentationbehavior and measuring the viscosity, the effects of dispersant concentration and pH value on thedispersion and stability of the suspensions were studied. The experiment results showed that the optimumconcentration of PMAA-NH4 and the optimum pH were 0.25-0.45 wt.%( by weight of the PZT powders)and 8.5-10, respectively. When the dispersant concentration was about 0.45 wt.%, and pH value wasabout 9.4, the stable PZT suspensions with high solids loading (up to 52 vol.%) and low viscosity (〈1Pa.s)was obtained for gelcasting. The microstructure of ceramic formed by gelcasting was denser and morehomogeneous than that by die pressing
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 182 (2000), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Escherichia coli INS33 was isolated from the urinary tract of an infected patient. It was resistant to ampicillin, chloramphenicol, spectinomycin, streptomycin, sulfafurazole, tetracycline and trimethoprim. PCR screening revealed the presence of a class 1 integron that harboured two new gene cassettes, designated dfrA17 and aadA5. The new dfrA17 cassette was 91% identical to the known dfrA7 cassette. The aadA5 cassette was 95% identical over the first 830 bp to aadA4, but lacked the IS26 element found at the 3′ end of this truncated cassette. Cloning and expression of the cassette region demonstrated that dfrA17 conferred high level resistance to trimethoprim but aadA5 conferred resistance to spectinomycin but not to streptomycin.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 146 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A 16-kb plasmid (pND859) was identified from Lactococcus lactis biovar. diacetylactis UK12922 which encodes phage resistance to the small isometric phage 712 when tested in L. lactis LM0230. The gene encoding phage abortive infection, designated abi-859, was localized on a 1.2-kb region which consists of an open reading frame (ORF) of 846 bp preceded by a potential ribosome-binding site and a putative promoter region. A helix-turn-helix region typical of DNA-binding motifs was identified near the N-terminal of the abi-859 product, suggesting a possible interaction with the phage DNA.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Annals of software engineering 7 (1999), S. 47-70 
    ISSN: 1573-7489
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract A Real-Time Architectural Specification (RAS) approach and its application to command and control (C2) systems are presented. Our objective is to establish a formal foundation that will enable us to integrate existing rich but fragmented formal techniques for system specification and verification into a practical and scaleable formal engineering method to support the design and development of highly reliable real-time distributed systems. The contribution of RAS is twofold: First, it provides a formal system that integrates system's timing requirements and requirements propagation into the process of architectural modeling and design in such a way that allows us to systematically enforce that the requirements are met in every step of the design process. Second, it offers an incremental and more scaleable approach for design modeling. These two features together make RAS a suitable model for the design of C2 systems. We further present an incremental method for verifying timing properties of an RAS model that helps to reduce the complexity of analysis both at a given design level and across different design levels.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of intelligent manufacturing 10 (1999), S. 485-502 
    ISSN: 1572-8145
    Keywords: Formal system design ; real-time systems ; time Petri nets ; real-time computational tree logic ; flexible manufacturing systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract An FMS is a typical real-time concurrent system composed of a number of computer-controlled machine tools, automated material handling and storage systems that operate as an integrated system under the control of host computer(s). The growing demand for higher performance and flexibility in these systems and the interlocking factors of concurrency, deadline-driven activities, and real-time decision making pose a significant challenge to FMS design, especially in terms of control and scheduling. A formal engineering approach that helps handle the complexity and dynamics of FMS modeling, design and analysis is needed. A real-time architectural specification (RAS) model and its application in the modeling of flexible manufacturing system (FMS) are presented. RAS combines mature operational and descriptive formal methods, in particular time Petri nets (TPN) and real-time computational tree logic (RTCTL), to form an integrated system model for architectural specification and analysis of real-time concurrent systems such as FMS. The contribution of RAS is twofold: First, it provides a formal system to systematically maintain a strong correlation between (real-time) requirements and design and to verify the conformance of the design to the requirements, which helps enhance traceability and thus to help us to achieve high assurance in design. Second, it offers better scalability in modeling and analysis, which provides an effective way to deal with complexity in the application of formal methods. These two features together make RAS a suitable model for the design of FMS.
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  • 8
    Publication Date: 2018-06-04
    Description: The stability of organic dyes against photobleaching is critical in single-molecule tracking and localization microscopy. Since oxygen accelerates photobleaching of most organic dyes, glucose oxidase is commonly used to slow dye photobleaching by depleting oxygen. As demonstrated here, pyranose-2-oxidase slows bleaching of Alexa647 dye by ∼20-fold. However, oxygen deprivation may pose severe problems for live cells by reducing mitochondrial oxidative phosphorylation and ATP production. We formulate a method to sustain intracellular ATP levels in the presence of oxygen scavengers. Supplementation with metabolic intermediates including glyceraldehyde, glutamine, and α-ketoisocaproate maintained the intracellular ATP level for at least 10 min by balancing between FADH2 and NADH despite reduced oxygen levels. Furthermore, those metabolites supported ATP-dependent synthesis of phosphatidylinositol 4,5-bisphosphate and internalization of PAR2 receptors. Our method is potentially relevant to other circumstances that involve acute drops of oxygen levels, such as ischemic damage in the brain or heart or tissues for transplantation.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 9
    Publication Date: 2019-08-12
    Description: Accurate mitosis depends on a surveillance system called the spindle assembly checkpoint. This checkpoint acts at kinetochores, which attach chromosomes to the dynamic tips of spindle microtubules. When a kinetochore is unattached or improperly attached, the protein kinase Mps1 phosphorylates kinetochore components, catalyzing the generation of a diffusible “wait” signal that delays anaphase and gives the cell time to correct the error. When a kinetochore becomes properly attached, its checkpoint signal is silenced to allow progression into anaphase. Recently, microtubules were found to compete directly against recombinant human Mps1 fragments for binding to the major microtubule-binding kinetochore element Ndc80c, suggesting a direct competition model for silencing the checkpoint signal at properly attached kinetochores. Here, by developing single-particle fluorescence-based assays, we tested whether such direct competition occurs in the context of native kinetochores isolated from yeast. Mps1 levels were not reduced on kinetochore particles bound laterally to the sides of microtubules or on particles tracking processively with disassembling tips. Instead, we found that Mps1 kinase activity was sufficient to promote its release from the isolated kinetochores. Mps1 autophosphorylation, rather than phosphorylation of other kinetochore components, was responsible for this dissociation. Our findings suggest that checkpoint silencing in yeast does not arise from a direct competition between Mps1 and microtubules, and that phosphoregulation of Mps1 may be a critical aspect of the silencing mechanism.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 10
    Publication Date: 2016-01-01
    Electronic ISSN: 2158-3226
    Topics: Physics
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