ISSN:
1573-4919
Keywords:
macrophage inflammatory protein
;
CC-CKR1
;
Chimeric MIP-1
;
MIP-1
;
binding and signaling by
;
chemotaxis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Abstract Human macrophage inflammatory protein-1α (hMIP-1α) and human macrophage inflammatory protein-1β (hMIP-1β) are chemokines involved in a diverse range of immunological effects. Both hMIP-1α and hMIP-1β are involved in the activation of monocytes and THP-1 cells probably through a common receptor(s). However, only hMIP-1α can bind to neutrophils with high affinity, presumably through CC-CKR1 (CKR1). Since the structure of these two proteins is highly conserved, non-conserved amino acids must define the disparate binding patterns that these two proteins exhibit. Measurements of binding, chemotaxis and calcium influx conducted with hMIP-1α and hMIP-1β chimeric proteins and mutants show that two amino acids (37K and 43L) are important in the binding and signaling of hMIP-1α through CKR1. Furthermore, we also show that mutations of the three charged amino acids at the C-terminus of hMIP-1α and hMIP-1β (amino acids 61, 65 and 67), do not adversely affect the binding to THP-1 cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006901109902
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