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  • 1
    Call number: ZSP-168-543 ; ZSP-168-543
    In: Berichte zur Polar- und Meeresforschung
    Type of Medium: Series available for loan
    Pages: 235 S. : graph. Darst.
    ISSN: 1618-3193
    Series Statement: Berichte zur Polar- und Meeresforschung 543
    Classification:
    Oceanology
    Language: English
    Location: AWI Reading room
    Location: AWI Reading room
    Branch Library: AWI Library
    Branch Library: AWI Library
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  • 2
    Call number: ZS-090(412) ; ZSP-168-412
    In: Berichte zur Polar- und Meeresforschung
    Type of Medium: Series available for loan
    Pages: 252 S.
    ISSN: 1618-3193
    Series Statement: Berichte zur Polar- und Meeresforschung 412
    Classification:
    Oceanology
    Language: German
    Location: Lower compact magazine
    Location: AWI Reading room
    Branch Library: GFZ Library
    Branch Library: AWI Library
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  • 3
    Publication Date: 2004-10-01
    Print ISSN: 0921-8971
    Electronic ISSN: 1573-5176
    Topics: Biology
    Published by Springer
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  • 4
  • 5
    Publication Date: 2004-01-01
    Print ISSN: 0964-5691
    Electronic ISSN: 1873-524X
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Published by Elsevier
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  • 6
    Publication Date: 2023-07-18
    Description: In the paper "Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults" (Cardoso et al. 2023) there was a need to compare artificial and natural seawater based on their nutrient composition and concentration. This experiment was performed to guarantee that the results from the other experiments in this paper were not caused by the different types of water, considering that before the start of these experiment the original cultures were growing in natural seawater. The artificial seawater (30 PSU) (Seequasal-Salz, Seequasal Salz Production and Trade GmbH, Münster, Germany) used in this experiment was made in the laboratory at the Alfred Wegener Institute (AWI), Bremerhaven, Germany, right before being analysed. The natural seawater (30 PSU ± 2 PSU) was collected by the Research Vessel Uthörn in the surrounding area of Helgoland, Germany. The process of collection of natural seawater was made every week to refill and supplement the tanks at AWI, Bremehaven. Therefore, the time of the collection and specific location are not known. The nutrient concentration analysis was done in October 2022 at AWI. Before the analysis both water types were pasteurized for 4 hours at 99 °C. The samples collected for analysis were filtered (0.2 µm) (Nalgene®, Nalge Nunc International, USA) and separated into falcon tubes (n = 3). The analysis was performed by an auto-analyser (SEAL Analytical, United Kingdom) and the concentrations of Phosphate, Ammonium, Nitrite and Nitrate were measured.
    Keywords: Algae cultivation; Ammonium; Antioxidant activity; Artificial seawater; AWI_Bremerhaven_ZFME; Bremerhaven, Germany; DATE/TIME; Date/time end; Date/time start; Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel; Laboratory; Laboratory experiment; Mak-Pak_Scale-Up; Nitrate; Nitrite; Number of measurements; Origin; Phosphate; Recirculating Aquaculture System; Refractometer, Atago, S-10E; Replicate; Salinity; Segmented flow analyzer, Seal Analytical; Temperature, water; Temperature data logger, Ebro, EBI 20-T1; Type of study; Ulva sp.; Vessel; Water description
    Type: Dataset
    Format: text/tab-separated-values, 174 data points
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  • 7
    Publication Date: 2023-07-18
    Description: In the paper "Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults" (Cardoso et al. 2023) the relative growth rates of germlings of different Ulva species and strains were measured to evaluate the impact of different salinity treatments. We hypothesised that, since their early stages, salinity impacts the relative growth rate of the seaweeds and that different strains are adapted to different salinities. With this data it was possible to use salinity as a tool for selecting the strain with the highest and determine the optimal salinity to grow its germlings under a nursery setting to supplement a large-scale production. Four strains were tested (U. lacinulata and U. linza from the NE-Atlantic and U. lacinulata and U. flexuosa from the Mediterranean). The NE- Atlantic strains were collected in the Óbidos Lagoon, Portugal in January 2021 and were cultivated in laboratory conditions in the Alfred Wegener Institute (AWI), Bremerhaven, Germany. The Mediterranean strains belong to an AWI collection and were isolated in 1986 and 1987 (U. flexuosa and U. lacinulata, respectively). The cultivation of the Mediterranean species with the purpose of using them for experiments started in June 2021. To recreate the conditions of a nursery system, the strains were grown previously and during the experiment in a medium of artificial seawater (Seequasal-Salz, Seequasal Salz Production and Trade GmbH, Münster, Germany) enriched with half-stregth Provasoli (PES; Provasoli 1968; modifications: HEPES-buffer instead of TRIS, double concentration of Na₂glycerophosphate; iodine enrichment following Tatewaki, 1966). Three germlings with similar size from each species and population were placed into multi-well plates and subjected to different salinity treatments (10, 15, 20 and 30 PSU) (n = 3). The germlings were cultivated for 3 weeks. Because of the small size of the germlings, measuring the fresh weight was not possible and, in this experiment, pictures of the germling development were taken every week and the area of the germlings was measured with the software Image J (Rasband 2012). The difference in size was later used to determine their relative growth rate.
    Keywords: Accession number, genetics; Algae cultivation; Antioxidant activity; Area; Artificial seawater; AWI_Bremerhaven_FunctEcologyLab; Bremerhaven, Germany; Concentration; DATE/TIME; Date/time end; Date/time start; DEPTH, water; Event label; Experiment duration; Image analysis NIH ImageJ; Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel; Laboratory; Laboratory experiment; LATITUDE; Light intensity; Light meter, LI-COR Biosciences GmbH, LI-250A; LONGITUDE; Mak-Pak_Scale-Up; Mediterranean Sea; MedSea_Ulva_1986; MedSea_Ulva_1987; NEAtlantic_Ulva_2021; North East Atlantic; Nutrient; Origin; Recirculating Aquaculture System; Refractometer, Atago, S-10E; Replicate; Salinity; Sample code/label; Sampling date; Species; Temperature, water; Temperature data logger, Ebro, EBI 20-T1; Treatment: light:dark cycle; Treatment: salinity; Type of study; Ulva sp.
    Type: Dataset
    Format: text/tab-separated-values, 3360 data points
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  • 8
    Publication Date: 2023-07-18
    Description: In the paper "Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults" (Cardoso et al. 2023) the antioxidant activity (AA) experiment aimed to test a previously selected strain, Ulva lacinulata from the NE-Atlantic, and evaluate the variations in AA under different salinity treatments. We hypothesized that by reducing the salinity level in the recirculating land-based system, it would be possible to optimize and increase the quality of the biomass being produced before it being harvested. The NE-Atlantic seaweed was collected in the Óbidos Lagoon, Portugal in January 2021 and was cultivated in laboratory conditions in the Alfred Wegener Institute (AWI), Bremerhaven, Germany. To recreate the conditions of a large-scale system, the strain was grown previously and during the experiment in a medium of artificial seawater (Seequasal-Salz, Seequasal Salz Production and Trade GmbH, Münster, Germany) enriched with a cheaper commercial fertilizer Blaukorn (COMPO SANA®, Germany). Twelve 1 L beakers were filled with artificial seawater at 4 different salinities (10, 15, 20 and 30 PSU), measured with a refractometer (Atago, Japan) (n = 3). Six discs (2 cm) of U. lacinulata were placed in each beaker and, at different times (0 h, 3 h, 24 h, 120 h, 192 h and 240 h) 1 disc was collected from each beaker to evaluate the antioxidant activity. The AA was determined by the ABTS Radical Cation Decolourisation Assay based on Re et al., 1999, and a Trolox standard curve was created by measuring the absorption of different Trolox concentrations in ethanol after being mixed with ABTS. The absorption of the samples at 734 nm was analysed with a microplate reader (Infinite 200 Microplate Reader, Tecan Trading AG, Männedorf, Switzerland) and the AA results obtained were given in Trolox Equivalent (in µg/mL).
    Keywords: Accession number, genetics; Algae cultivation; Antioxidant activity; Antioxidant capacity, in Trolox Equivalents; Artificial seawater; AWI_Bremerhaven_FunctEcologyLab; Bremerhaven, Germany; Concentration; DATE/TIME; Date/time end; Date/time start; DEPTH, water; Event label; Experiment duration; Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel; Laboratory; Laboratory experiment; LATITUDE; Light intensity; Light meter, LI-COR Biosciences GmbH, LI-250A; LONGITUDE; Mak-Pak_Scale-Up; Microplate reader, Tecan Trading AG, Infinite 200; NEAtlantic_Ulva_2021; North East Atlantic; Nutrient; Origin; Recirculating Aquaculture System; Refractometer, Atago, S-10E; Replicate; Salinity; Species; Temperature, water; Temperature data logger, Ebro, EBI 20-T1; Treatment: light:dark cycle; Treatment: salinity; Type of study; Ulva sp.
    Type: Dataset
    Format: text/tab-separated-values, 1147 data points
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  • 9
    Publication Date: 2023-07-18
    Description: In the paper "Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults" (Cardoso et al. 2023) it was evaluated the impact of different nutrient sources on the relative growth rates (RELATIVE GROWTH RATE) of four different Ulva strains during their adult stage. Previously to this experiment, two experiments were performed to evaluate the of germlings and adults of the four Ulva strains. Because the aim of these experiments was to reproduce the conditions of a large-scale production and a small-scale nursery, the adult material was supplemented with a cheaper commercial fertilizer Blaukorn (COMPO SANA®, Germany) while the germlings (in the nursery set-up) were supplemented with half-strength Provasoli (PES; Provasoli 1968; modifications: HEPES-buffer instead of TRIS, double concentration of Na₂Glycerophosphate; iodine enrichment following Tatewaki, 1966). Therefore, this experiment was performed to guarantee that the use of different nutrient sources would not impact the results of the former experiments. Four strains were tested (U. lacinulata and U. linza from the NE-Atlantic and U. lacinulata and U. flexuosa from the Mediterranean). The NE- Atlantic strains were collected in the Óbidos Lagoon, Portugal in January 2021 and it was cultivated in laboratory conditions in the Alfred Wegener Institute (AWI), Bremerhaven, Germany from that moment on. The Mediterranean strains belong to an AWI collection and were isolated in 1986 and 1987 (U. flexuosa and U. lacinulata, respectively). The cultivation of the Mediterranean species with the purpose of using them for experiments started in June 2021. Fresh thalli from each species and population were placed into 1 L glass beakers and subjected to one of the two treatments: water enriched with commercial fertilizer Blaukorn or with half-strength Provasoli (n = 3). The experiment ran for 3 weeks. The fresh weight was measured once a week by collecting the seaweed and removing the excess water with absorbent paper three times before weighing the samples (Sartorius, Germany). Every time, each sample was measured 3 times. Based on the fresh weight measured each week, it was possible to calculate the of each strain throughout the experiment.
    Keywords: Accession number, genetics; Algae cultivation; Analytical Balance, Sartorius, LA310S; Antioxidant activity; Artificial seawater; AWI_Bremerhaven_FunctEcologyLab; Biomass, wet mass; Bremerhaven, Germany; Concentration; DATE/TIME; Date/time end; Date/time start; DEPTH, water; Event label; Experiment duration; Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel; Laboratory; Laboratory experiment; LATITUDE; Light intensity; Light meter, LI-COR Biosciences GmbH, LI-250A; LONGITUDE; Mak-Pak_Scale-Up; Mediterranean Sea; MedSea_Ulva_1986; MedSea_Ulva_1987; NEAtlantic_Ulva_2021; North East Atlantic; Number of measurements; Origin; Recirculating Aquaculture System; Refractometer, Atago, S-10E; Replicate; Salinity; Sample code/label; Sampling date; Species; Temperature, water; Temperature data logger, Ebro, EBI 20-T1; Treatment: light:dark cycle; Treatment: nutrients; Type of study; Ulva sp.
    Type: Dataset
    Format: text/tab-separated-values, 5328 data points
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  • 10
    Publication Date: 2023-07-18
    Description: In the paper "Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults" (Cardoso et al. 2023) the relative growth rates of adult thalli of different Ulva species and strains were measured to evaluate the impact of different salinity treatments. We hypothesised that salinity impacts the of the seaweeds and that different strains are adapted to different salinities. With this data we aimed to use salinity as a tool for selecting the strain with the highest and determine the optimal salinity to grow it in the future in a recirculating land-based system. Four strains were tested (U. lacinulata and U. linza from the NE-Atlantic and U. lacinulata and U. flexuosa from the Mediterranean). The NE- Atlantic strains were collected in the Óbidos Lagoon, Portugal in January 2021 and were cultivated in laboratory conditions in the Alfred Wegener Institute (AWI), Bremerhaven, Germany. The Mediterranean strains belong to an AWI collection and were isolated in 1986 and 1987 (U. flexuosa and U. lacinulata, respectively). The cultivation of the Mediterranean species with the purpose of using them for experiments started in June 2021. To recreate the conditions of a large-scale system, the strains were grown previously and during the experiment in a medium of artificial seawater (Seequasal-Salz, Seequasal Salz Production and Trade GmbH, Münster, Germany) enriched with a cheaper commercial fertilizer Blaukorn (COMPO SANA®, Germany). A uniform amount of fresh thalli from each species and population were placed into 1 L glass beakers with salinities of 10, 15, 20 and 30 PSU (each condition n = 3) and cultivated over 3 weeks. The fresh weight was measured once a week by collecting the seaweed and removing the excess water with absorbent paper three times before weighing the samples (Sartorius, Germany). Every time, each sample was measured 3 times. Based on the fresh weight measured each week, it was possible to calculate the of each strain throughout the experiment.
    Keywords: Accession number, genetics; Algae cultivation; Analytical Balance, Sartorius, LA310S; Antioxidant activity; Artificial seawater; AWI_Bremerhaven_FunctEcologyLab; Biomass, wet mass; Bremerhaven, Germany; Concentration; DATE/TIME; Date/time end; Date/time start; DEPTH, water; Event label; Experiment duration; Industrietaugliche Verfahrensoptimierung zur Herstellung einer nachhaltigen Verpackungslösung aus Makroalgen für den Lebensmittel-Handel; Laboratory; Laboratory experiment; laboratory study; LATITUDE; Light intensity; Light meter, LI-COR Biosciences GmbH, LI-250A; LONGITUDE; Mak-Pak_Scale-Up; Mediterranean Sea; MedSea_Ulva_1986; MedSea_Ulva_1987; NEAtlantic_Ulva_2021; North East Atlantic; Number of measurements; Nutrient; Origin; Recirculating Aquaculture System; Refractometer, Atago, S-10E; Replicate; Salinity; Sample code/label; Sampling date; Species; Temperature, water; Temperature data logger, Ebro, EBI 20-T1; Treatment: light:dark cycle; Treatment: salinity; Type of study; Ulva sp.
    Type: Dataset
    Format: text/tab-separated-values, 10656 data points
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